ICAM-1协同参与TNFRI介导的TM-TNF-α杀瘤作用  

Participation of ICAM-1 in the cytotoxicity of TM-TNF-α mediated by TNF receptor I

作　　者：郑芳[1] 石文芳[1] 冯玮[1] 姜晓丹[1] 徐勇[1] 范舫 李卓娅[1] 

机构地区：[1]华中科技大学同济医学院免疫学系,湖北武汉430030

出　　处：《细胞与分子免疫学杂志》2004年第1期42-44,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .39630 32 0 ;39870 71 3)

摘　　要：目的 :寻找协同参与TM TNF α杀瘤作用的膜表面分子 ,探讨TM TNF α杀瘤及两型TNF α生物学效应差异的分子机制。方法 :利用抗体封闭实验及RT PCR ,检测ICAM 1及VCAM 1对TNFR1或TNFRII介导的TM TNF α杀瘤效应是否具有协同作用。结果 :封闭表达TNFRI的MCF 7细胞表面的ICAM 1,可显著降低TM TNF α对其杀伤的作用 ;而封闭VCAM 1无明显效应。封闭表达TNFRII的HL 6 0细胞表面的ICAM 1或VCAM 1,均对TM TNF α的杀伤作用无影响。结论 :ICAM 1对于TM TNFAIM: To identify membrane-associated molecules involved in the tumoricidal cytotoxicity of TM-TNF-α and to explore the molecular mechanism underlying the tumoricidal cytotoxicity of TM-TNF-α and differences of biological effects between TM-TNF-α and sTNF. METHODS: Antibody blocking test and RT-PCR were used to evaluate the role of ICAM-1 and VCAM-1 in the cytotoxicity of TM-TNF-α mediated by TNFR I or TNFR II. RESULTS: After the ICAM-1 expressed on the membrane of MCF-7 cells(expressing TNFRI) was blocked, the cytotoxicity of TM-TNF-α to this cell lines decreased significantly, while blocking VCAM-1 had no effect. Blocking ICAM-1 or VCAM-1 expressed on the membrane of HL-60 cells(expressing TNFRII)had no significant effects on the cytotoxicity of TM-TNF-α to this cell lines. CONCLUSION: ICAM-1 participates in the cytotoxicity of TM-TNF mediated by TNFRI.

关 键 词：ICAM-1 TM-TNF-Α 协同 杀瘤作用 

分 类 号：R392.11[医药卫生—免疫学]