烧伤大鼠休克期红细胞趋化因子受体结合活性的变化  

The change of the erythrocyte chemokine receptor binding activity in the shock stage of burn rats

作　　者：陈晓东[1] 王顺宾[1] 吴伯瑜[1] 江琼[1] 

机构地区：[1]福建医科大学附属协和医院福建省烧伤研究所,福建福州350001

出　　处：《细胞与分子免疫学杂志》2004年第1期58-60,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省科学技术厅重点课题资助 (No.2 0 0 2Y0 1 6);福建省卫生厅青年基金课题资助 (No.2 0 0 3 1 2 1 )

摘　　要：目的 :探讨烧伤大鼠休克期红细胞趋化因子受体 (ECKR)结合活性的变化。方法 :SD大鼠随机分成烧伤组和对照组 ;烧伤组大鼠体表总面积 (TBSA)的 30 %为Ⅲ度烫伤。应用ELISA法 ,以IL 8为配体检测烧伤后休克期不同时间点ECKR的结合活性。结果 :伤后 0 .5h起 ,大鼠ECKR的结合活性均明显下降 ,并维持至伤后 4 8h ,与对照组相比较差异显著 (P <0 .0 1)。不同时间点相比较 ,自伤后 2h起大鼠ECKR的结合活性呈逐渐下降趋势 ,至伤后 2 4h达最低值 ;伤后 4 8h ,大鼠ECKR的结合活性则有明显升高 ,与对照组相比较差异均显著 (P <0 .0 5～ 0 .0 1)。结论 :烧伤后ECKR的结合活性明显下降 。AIM: To investigate the change of the erythrocyte chemokine receptor(ECKR) binding activity in the shock stage of burn rats. METHODS: SD rats were randomly divided into two group, burn and control groups. In the burn group rats, 30% total body surface area (TBSA)were scalded to Ⅲ degree. The binding activity of rat ECKR in the shock stage was detected by ELISA using IL-8 as ligand at various time points (0.5, 2, 4, 8, 16, 24 and 48 hours) after burn. RESULTS: Compared with control group, the binding activity of rat ECKR declined significantly half an hour after burn and maintained at low level for 48 hours (P<0.01). The comparison of the binding activity of rat ECKR at various time points after burn indicated that the binding activity declined gradually from 2 hours (P<0.01-0.05), reached lowest value 24 hrs, and then rose significantly 48 hrs after burn (P<0.01). CONCLUSION: The ECKR binding activity declined significantly after burn, suggesting erythrocytes may participate in the regulation of chemokines and play some role in the inflammation.

关 键 词：烧伤 红细胞趋化因子受体 白细胞介素-8 

分 类 号：R644[医药卫生—外科学]