通痹灵总碱对T细胞内Th1型细胞因子表达的影响  

Effects of total alkaloids of Tongbiling prescription on Th1 type cytokine expression in T lymphocytes

作　　者：李晓娟[1] 陈光星[2] 刘良[3] 王培训[1] 胡英杰[4] 曾耀英[5] 陈纪藩[2] 

机构地区：[1]广州中医药大学免疫研究室,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405 [3]香港浸会大学中医药学院 [4]广州中医药大学热带病研究所,广东广州510405 [5]暨南大学组织移植与免疫教育部重点实验室

出　　处：《细胞与分子免疫学杂志》2004年第1期113-116,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金重点项目资助 (No.39930 2 0 )

摘　　要：目的 :研究通痹灵总碱 (TBL)对T细胞中Th1型细胞因子表达的影响 ,并揭示其抗炎机制 ,为临床用药提供理论依据。方法 :分离小鼠肠系膜淋巴结细胞 ,加入不同浓度的TBL作用 1h ,再加多克隆刺激剂佛波醇酯 (PDB)和离子酶素 ,继续培养 4h收获细胞 ,进行双色荧光素标记 ,以流式细胞术对Th1型细胞因子INF γ和TNF α的表达进行分析。结果 :2 0 0mg/L 和 10 0mg/L的TBL ,能显著抑制T细胞内INF γ和TNF α的表达。结论 :TBL可抑制Th1型炎症性细胞因子的表达 。AIM: To study the effects of total alkaloid of Tongbiling prescription(TBL) on Th1 type cytokine expression in T cells in order to elucidate the anti-inflammatory mechanism of TBL. METHODS: The lymphocytes were isolated from mouse mesenteric lymph nodes and cultured in vitro. Various concentrations of TBL were added to the culture followed by phorbol ester and inomycin treatment and then incubated for another 4 hours. The expressions of IFN-γ and TNF-α in the lymphocytes were analyzed by flow cytometry. RESULTS: 200 mg/L and 100 mg/L TBL could obviously inhibit IFN-γ and TNF-α expressions in T lymphocytes. CONCLUSION: Inhibiting Th1 cytokine expression may be one mechanism by which TBL can treat rheumatoid arthritis.

关 键 词：通痹灵总碱 INF-Γ TNF-Α TH1细胞 类风湿关节炎 

分 类 号：R285.5[医药卫生—中药学]