褪黑素受体亚型Mel1a和Mel1b在大鼠中枢神经系统的分布差异——原位杂交研究  

THE DISTRIBUTION DIFFERENCE BETWEEN Mel 1a AND Mel 1b MELATONIN RECEPTORS IN THE CENTRAL NERVOUS SYSTEM OF RATS

作　　者：徐海伟[1] 黎海蒂[1] 吴旋[1] 范晓棠[2] 龚发云[1] 

机构地区：[1]第三军医大学生理学教研室,重庆400038 [2]第三军医大学神经生物学教研室,重庆400038

出　　处：《解剖学报》2003年第2期129-134,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 10 0 0 87) ;军队十五青年基金资助项目 ( 0 1Q0 99)

摘　　要：目的　研究Mel 1a、Mel 1b褪黑素膜受体在大鼠中枢神经系统的表达及分布差异。　方法　原位杂交组织化学。　结果　 (1)Mel 1amRNA阳性细胞主要分布于海马、大脑皮层、视上核、室旁核、视交叉上核、橄榄核、小脑皮层、小脑顶核、脊髓前角、面神经核、巨细胞网状核、纹状皮质、三叉神经核等。 (2 )Mel 1bmRNA阳性细胞主要分布于小脑皮层和顶核、球状核、栓状核、海马、大脑皮层、脊髓前角、视上核和交叉上核。　结论　Mel 1amRNA在中枢分布广泛、含量丰富 ,而Mel 1bmRNA在中枢分布较局限。在海马和大脑皮层 。Objective To exam the expression and the distribution difference between melatonin membrane receptor subtype Mel 1a and Mel 1b in the central nervous system of rats. Methods In situ hybridization technique was used. Results (1)The Mel 1a mRNA positive cells were mainly detected in the hippocampus,cerebral cortex,supraoptic nucleus,paraventricular nucleus,suprachiasmatic nucleus,inferior olivary nucleus,cortex and fastigial nucleus of cerebellar,ventral horn of the spinal cord,facial nerve nucleus,gigantocellular reticular nucleus,striatum cortex and trigeminal nerve nucleus,etc.(2)The Mel 1b mRNA positive cells were mainly observed in the cerebellar cortex,fastigial nucleus,global nucleus,emboliform nucleus of the medullaris cerebelli,hippocampus,cerebral cortex,ventral horn of the spinal cord,supraoptic nucleus and suprachiasmatic nucleus.Conclusion\ Mel 1a mRNA positive neurons were abundant and distributed widely in the CNS,while Mel 1b mRNA\|positive neurons distributed comparatively localized.However,the hippocampus and the cortex were two regions which were rich in both Mel 1a and Mel 1b mRNA positive neurons.\;[

关 键 词：褪黑素受体 Mel1a Mel1b 中枢神经系统 分布差异 原位杂交 

分 类 号：R338[医药卫生—人体生理学]