缺血再灌注损伤皮瓣组织中金属硫蛋白含量变化  

Changes of metallothionein contents in rat flap with ischemia -reperfusion injury

作　　者：王冬艳[1] 郭杰[1] 关德宏[1] 肖志波[1] 唐朝枢[2] 

机构地区：[1]哈尔滨医科大学附属第二医院整形外科,150086 [2]北京大学医学部生理学系

出　　处：《中华医学美学美容杂志》2003年第5期296-298,共3页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：黑龙江省攻关项目(G99C2 0 1 0 2 )

摘　　要：目的　观察缺血再灌注损伤皮瓣组织中金属硫蛋白 (metallothionein ,MT)的含量变化。方法　 16只大鼠随机分为对照组 (n =8)和缺血再灌注损伤组 (n =8)。在大鼠腹壁浅血管为蒂的岛状皮瓣缺血再灌注损伤模型上 ,用10 9Cd血红蛋白饱和法和硫代巴比妥酸法测定皮瓣组织中MT及丙二醛(MDA)含量 ,比色法测定皮瓣组织过氧化物酶 (MPO )活性。结果　缺血再灌注损伤组在缺血 8h、再灌注 12h、2 4h时皮瓣组织中MDA水平分别较对照组高 41.7%、111.4%、13 5.7% ,MPO水平分别较对照组高 72 .1%、2 18.9%、2 96.0 % ,MT含量分别较对照组高 42 .6%、52 .8%、10 2 .3 % (P <0 .0 5或P<0 .0 1)。结论　皮瓣组织中MT含量增多 。Objective To observe the changes of metallothionein (M T) in the rat flap with ischemia-reperfusion injury. Methods A ri ght lower abdominal island flap was created in Wistar rats . 16 rats were random ly divided into two groups: the control ( n =8) and the ischemia-reperfusion injury group ( n =8). The contents of MT and malondialdehyde (MDA) were assaye d by 109 Cd-hemoglobin saturation and thiobarbituric acid (TBA) methods. Th e activity of myeloperoxidase (MPO) was measured by colorimetry. Results At ischemia 8 h, reperfusion 12 h and 24 h, the level of MDA in rat i schemia-reperfusion injury group increased 41.7 %, 111.4 %, and 135.7 %, the ac tivity of MPO increased 72.1 %, 218.9 % and 296.0 %, the content of MT increased 42.6 %, 52.8 % and 102.3 %, respectively, compared with the control group ( P <0.05 or P <0.01) .Conclusion The change of MT in rat flap may be related to ischemia-reperfusion injury.

关 键 词：缺血再灌注损伤 皮瓣组织 金属硫蛋白 MT 手术 心肌缺血 

分 类 号：R622[医药卫生—整形外科]