培育具有安全选择标记或无选择标记的转基因植物  

Breeding Transgenic Plants with Safe or No Selective Markers

作　　者：李晓兵[1] 陈彩艳[1] 翟文学[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《遗传》2003年第3期345-349,共5页Hereditas（Beijing)

基　　金：国家自然科学基金(30270857)资助

摘　　要：转基因植物中选择标记的安全性已成为植物基因工程研究的热点之一。从两个方面可以解决转基因植物中的选择标记问题。一是选用安全的正向选择标记,主要是与糖代谢和激素代谢相关的基因。二是构建能去除选择标记基因的转化系统,主要有共转化系统、双T DNA边界载体系统、位点特异性重组系统和转座子系统等。这些植物基因工程的方法将有助于培育安全的转基因植物。The biosafety of selective markers in transgenic plants has been a hot spot in the field of plant genetic engineering.To solve the problem of selective markers in the transgenic plants,two means of producing transgenic plants have been developed.One is the utilization of biosafe positive selective markers which are genes mainly related to metabolism of auxins and carbohydrates.The other is the establishment of transformation systems allowing marker genes to be eliminated from the transgenic plants,which include cotransformation,double TDNA border vectors,sitespecific recombination and transposition.All these approaches of plant genetic engineering will benefit breeding transgenic plants with biosafety.

关 键 词：转基因植物 生物安全性 选择标记 植物基因工程 糖代谢 激素代谢 转化系统 

分 类 号：Q943.2[生物学—植物学]