新疆哈萨克族人血管紧张素原基因M235T多态性与原发性高血压相关性研究  

Relationship between the Polymorphism of Angiotensinogen Gene M235T and Human Essential Hypertension in Kazakans of Xinjiang

作　　者：李南方[1] 周玲[1] 殷晓娟[2] 吴卫东[3] 杨忠礼[4] 普雄明[3] 石岩[3] 李红建[2] 王新玲[1] 王君[1] 张德莲[1] 祖合热[1] 欧阳玮进[1] 布克力[1] 周克明[1] 成秋艳[1] 努尔古丽[1] 朱登浩 马拉提 

机构地区：[1]新疆自治区人民医院高血压研究所,乌鲁木齐830001 [2]新疆医科大学临床医学院 [3]新疆自治区人民医院皮肤科 [4]新疆自治区人民医院检验科 [5]新疆福海县人民医院

出　　处：《科学技术与工程》2004年第3期193-197,共5页Science Technology and Engineering

基　　金：国家 863预研课题 (国科生字2 0 0 0 112号 );国家自然科学基金 ( 0 3 0 3 0 2 0 1)资助

摘　　要：探讨血管紧张素原 (AGT)基因M2 35T分子变异与哈萨克人原发性高血压 (EH)的关系 ,采用多聚酶链式反应法及限制性片段长度多态性技术 (PCR RFLP)对 132例EH(EH组 )及 75例正常者 (正常对照组 )进行AGT基因M2 35T多态性检测。新疆哈萨克族原发性高血压AGT基因型频率为TT :0 ,TM :4 1.6 7% ,MM :5 8.33% ;T2 35与M2 35等位基因频率分别 2 0 .83% ,79.17%。而对照组其相应频率分别为 0 ,4 6 .6 7% ,5 3.33% ;2 3.33% ,76 .6 7% ,两组相比无显著性差异 (P >0 .0 5 )。按性别分组后 ,男女等位基因频率两组相比亦无显著性差异 (P >0 .0 5 )。证明AGT基因M2To investigate the relationship between the M 235T allele polymorphism of angiotensinogen (AGT) gene and the essential hypertension in Kazakans of Xinjiang, polymerase Chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods were used to detect the M235T polymorphism of AGT gene in Kazakans including 132 patients and 75 normotensive controls. Results are that the genotype frequencies of M235T were TT 0,TM41.67%, MM 58.33% respectively; T235 and M235 allele frequencies were 20.83%, 79.17% respectively in hypertension group. While the corresponding frequencies were 78.38%,21.62%,0%;89.19%,10.81% respectively in control group. There was no significant difference in 235MM genotype and 235M allele frequencies of angiotensinogen between hypertension and control groups (P>0.05). In addition, T235 and M235 allele frequencies had also no statistic significance in the usbgroups classified according to sex (P>0.05). It is conclused there was no significant association found between the M235T allele polymorphism of AGT gene and essential hypertension in Kazakans of Xinjiang.

关 键 词：新疆 哈萨克族人 血管紧张素原基因 M235T 多态性 原发性高血压 

分 类 号：R544.1[医药卫生—心血管疾病]