中药材厚朴的随机扩增多态性DNA指纹图谱研究  

Studies on Random Amplified Polymorphic DNA Fingerprinting of Cortex Magnoliae Officinalis

作　　者：刘文生[1] 朱建明[2] 何斌[1] 苏应娟[2] 

机构地区：[1]广东省人民医院,广州510080 [2]中山大学生命科学学院,广州510275

出　　处：《中药材》2004年第3期164-169,共6页Journal of Chinese Medicinal Materials

基　　金：广东省中医药管理局资助项目

摘　　要：目的 :鉴别中药材厚朴及其伪品、混淆品。方法 :采用随机引物扩增多态性DNA(RAPD)技术扩增植物基因组DNA样品。结果 :此种分子生物学鉴别方法可以获得清晰可靠的DNA指纹图谱 ,根据琼脂糖凝胶上显示的DNA带型差异可准确区分出厚朴、凹叶厚朴和其伪品、混淆品。结论 :RAPD方法可以准确、快速、简便地鉴定中药材厚朴及其伪、混品 ,尤其在正确引种方面具有很高的价值。Objective: To identify Chinese traditional medicine(CTM)'Hou-pu' (Cortex Magnoliae Officinalis), its counterfeits and substitues. Methods: Total genomic DNA samples of ten plant species were amplified by randomly amplified polymorphic DNA(RAPD). Results: Ten samples were able to be distinguished through their amplified DNA banding patterns on the agarose gels after electrophoresis. Conclusion: RAPD is able to identify ' Hou-pu', its counterfeits and substitutes quickly and truly, which is also quite valuable for correctly introducing plant.

关 键 词：中药材 厚朴 随机扩增 多态性 DNA指纹图谱 

分 类 号：R282[医药卫生—中药学]