钉螺肝脏线粒体的氧化磷酸化作用  

OXIDATIVE PHOSPHORYLATION OF LIVER MITOCHONDRIA IN ONCOMELANIA SNAIL

作　　者：宋庚明[1] 王根法[1] W.Becker 

机构地区：[1]中国预防医学科学院寄生虫病研究所(世界卫生组织血吸虫病,疟疾和丝虫病合作中心),上海200025 [2]汉堡大学动物学研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1992年第2期113-116,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：德国大众汽车公司基金会部分资助

摘　　要：应用氧电极技术及比色法测得钉螺肝脏线粒体具有氧化磷酸化作用;二磷酸腺苷(ADP)可使线粒体氧化反应从0.187增至0.318μmol O_2/mg蛋白·20min。有氧代谢三核酸循环的中间物可增加钉螺肝脏线粒体氧化反应0.353—0.444μmol O_2/mg蛋白·20min。线粒体氧化反应同时偶联磷酸化反应。钉螺肝脏具有三磷酸腺苷酶(ATPase)活力。二硝基苯酚及溴乙酰胺可明显抑制钉螺线粒体氧化磷酸化作用,氧化反应分别从0.431降至0.245及0.282μmol O_2/mg蛋白·20min。溴乙酰胺并不抑制ATPase活力。Oxidative phosphorylation of liver mitochondria in Oncomelania snail was separately detected by using oxygen electrode and spectrophotometer.ADP increased oxidative reaction of liver mitochondria from 0.187 to 0.318μmol O2/mg protein·20 min.When certain substrates of citric acid cycle were added to liver mitochondria of Oncoomelania snail,we found that oxidative phosphorylation increased to 0.353-0.444μmol O2/mg protein·20 min.ATPase was detected in the liver of Oncomelania snail.The oxidative phosphorylation of mitochondria in Oncomelania snail could be markedly inhibited by DNP and molluscicide bromoacetamide,but the latter didn't show the inhibition of ATPase.(Figs.1,2)

关 键 词：钉螺 线粒体 氧化磷酸化 血吸虫 

分 类 号：R383.24[医药卫生—医学寄生虫学]