PC12细胞化学缺氧复氧损伤与缺氧诱导因子1表达的关系(英文)  

Relationship between chemical hypoxia-reoxygenation injury and hypoxia inducible factor 1 expression in PC12 cells

作　　者：王斌[1] 倪永兵[1] 王娟[1] 李华[1] 严虹[1] 戈应滨[1] 肖继皋[1] 

机构地区：[1]南京医科大学药理学系,江苏南京210029

出　　处：《中国神经科学杂志》2004年第2期99-104,共6页

基　　金：国家自然科学青年基金 (3 0 0 0 0 2 0 7) ;江苏省教育厅重点项目 (KJB3 10 0 0 1) ;江苏省高校高新技术产业化项目 (JH0 1 0 5 0 )

摘　　要：目的　研究缺氧诱导因子 1(HIF1)在PC12细胞化学缺氧复氧损伤中的作用。方法在培养液中加入和去除氯化钴模拟化学缺氧和复氧 ,以乳酸脱氢酶 (LDH)漏出和细胞超微结构改变作为细胞损伤指标 ,观察化学缺氧和复氧后不同时间细胞损伤和HIF1α蛋白变化。结果　 在氯化钴模拟化学缺氧实验中 ,LDH漏出明显增加 ,8h达高峰 ,随后逐渐下降 ,电镜结果与LDH改变相一致 ,HIF1α蛋白表达在化学缺氧后 2 ,4 ,8和 12h均明显增加 ,8h达高峰 ,提示化学缺氧 8h后细胞损伤逐渐减轻可能与HIF1α蛋白水平升高有关。在模拟复氧实验中 ,LDH和细胞形态学改变都显示化学复氧 8h细胞损伤最为严重 ,而HIF1α蛋白表达在化学复氧 4和 8h均明显下降 ,提示细胞化学复氧损伤可能与HIF1α蛋白水平下降有关。 结论　HIF1对神经细胞化学缺氧复氧损伤具有保护作用。Objective To study the possible relationship between hypoxia inducible factor 1 (HIF1) and neuronal injury during chemical hypoxia and reoxygenation. Methods Chemical hypoxia and reoxygenation were simulated by the addition and the removal of CoCl_ 2 respectively in PC12 cells.Cell injury, represented by LDH leakage and morphological changes,and HIF1 α protein expression checked with a confocal scanning laser microscope were investigated and evaluated upon different periods of chemical hypoxia and reoxygenation. Results In PC12 cells treated with CoCl_ 2 , the leakage of LDH were increased significantly since 2 h, peaked at 8 h, then declined at 12 and 16 h. The ultrastructure changes of PC12 cells correlated with the results in LDH. Treatment with CoCl_ 2 were found to apparently elevate HIF1 α protein expression in 2,4,8 and 12 h. The relief of cell injury after 8 h appeared to be a result of the elevation of HIF1 α protein levels. In PC12 cells treated with the removal of CoCl_ 2 , LDH levels were increased significantly in contrast to those in cells with continuous chemical hypoxia at 8 h after chemical reoxygenation. The morphological alterations of PC12 cells were consistent with the results in LDH. HIF1 α protein expression were dramatically declined at 4 and 8 h after reoxygenation. The aggravation of cell injury after reoxygenation appeared to be a result of the decrease of HIF1 α protein expression. Conclusion HIF1 could be the key role in the protection against the neuronal injury after chemical hypoxia-reoxygenation.

关 键 词：PC12细胞 化学缺氧复氧损伤 缺氧诱导因子1 表达 

分 类 号：Q786[生物学—分子生物学]