中间球海胆雌核发育单倍体胚胎的初步研究  

The embryonic development in gynogenesis monoploid in sea urchin (Strongylocentrotus intermedius)

作　　者：丁君[1] 常亚青[1] 曹学彬[1] 张玉勇[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室

出　　处：《大连水产学院学报》2004年第1期10-15,共6页Journal of Dalian Fisheries University

基　　金：国家"863"项目资助(2002AA628170)

摘　　要：采用不同剂量(30～330mJ/cm2)的紫外线对中间球海胆Strongylocentrotusintermedius精子进行照射失活,获得了雌核发育的单倍体胚胎。测定了照射组与对照组的受精率、单倍体诱导率、2～4细胞期的畸形率和破膜囊胚成活率,探明了精子灭活的紫外线适宜剂量为270mJ/cm2;并进行了早期胚胎地衣红压片观察和早期囊胚染色体数分析,观察到照射组早期胚胎的精核具有DCB小体,对照组早期囊胚的染色体数为2n=42,照射组雌核发育单倍体胚胎的染色体数为n=21。The monoploid embryo of gynogenesis in sea urchin (Strongylocentrotus intermedius) was obtained from spermatozoa by various intensities of ultraviolet radiation (from 30 mJ/cm^(2)to 330 mJ/cm^2). Fertilization rate, incidence of monoploid, incidence of deformed in 2-celled stage and 4-celled stage, and survival rate in blastula stage were determined in sea urchin embryo during the gynogenesis. It was found that the optimal intensity of ultraviolet radiation was 270 mJ/cm^2, at which the sperm nucleus condensed into chromatin-DCB(Density Chromatin Body) in fertilized eggs and in 2-4 celled stage in treatment group. In control group, the chromosome number in the monoploid embryo was 2n=42, while, in treatment group, the chromosome number was n=21.

关 键 词：中间球海胆 雌核发育 单倍体胚胎 紫外线 精子遗传物质 照射剂量 受精 孵化 染色体制备 

分 类 号：Q958.83[生物学—动物学] Q959.268.3