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出 处:《生态学杂志》2004年第2期80-85,共6页Chinese Journal of Ecology
基 金:国家重点基础研究发展规划项目 ( 0 0 1CB10 90 0 4);国家自然科学基金项目 ( 3 9970 5 0 7);教育部全国优秀博士学位论文专项资助项目( 199944 )
摘 要:对近年来《Nature》刊登的、并在全球范围内引起转基因玉米生态安全争论的两个事件进行了回顾 ,一是 1999年由Losey等引发的大斑蝶事件 ,另一是 2 0 0 1年由Quist等引发的转基因渗入墨西哥当地玉米品种事件。同时对其它学者分别就这两个事件进行的更为细致、严密的研究进行了全面的回顾 ,总结发现 :Losey等实验设计没有合理的对照和具体的供试花粉剂量及毒素浓度 ,而Quist等则因为反向PCR引物设计的不当把从墨西哥当地玉米品种K1、A3中扩增出的产物误认为与转基因玉米品种中的adh1基因同源。可见两个事件在实验设计上均存在一定的缺陷。Two controversial events, pubulished in《Nature》, on the ecological safety of transgenic corn, had raised extensive controversies around the world in recent years. One was the controversy of monarch butterfly Danaus plexippus caused by Losey's et al. in 1999, and the other was transgenic DNA introgressed into traditional corn landraces in Mexico caused by Quist et al. in 2001. Also, more meticulous and rigorous studies done by different researchers on the two events were comprehensively reviewed. The results showed that there were no proper control, specific dosage of tested pollen and concentration of toxin in Losey's et al., while for Quist et al., they confused the amplifications of reverse PCR of landraces K1, A3 in Mexico with adh1 gene in commercial transgenic corn caused by their incorrect reverse PCR primers. As a result, there were some faults in experiment designs of the two controversial events.
分 类 号:S188[农业科学—农业基础科学]
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