组织培养快速繁殖龙翅海棠  

Rapid Propagation of Drgeon Begonia by Tissue Culture

作　　者：黄燕芬[1] 范成五[1] 唐丽[1] 

机构地区：[1]贵州省农业科学院土壤肥料研究所,贵阳550006

出　　处：《种子》2004年第4期41-42,共2页Seed

摘　　要：龙翅海棠的组培可以幼嫩叶片作外植体 ,通过诱导形成愈伤组织分化不定芽的途径 ,建立无菌系 ,获得再生植株。最适诱导分化培养基为 MS+1mg/ L 6 - BA+0 .1mg/ L NAA+3%蔗糖 +0 .6 %琼脂 ;最适增殖培养基为 :MS+0 .8mg/L 6 - BA+0 .0 8mg/ L NAA+3%蔗糖 +0 .6 %琼脂 ;最适生根培养基为 :1/ 2 MS+0 .0 8mg/ L NAA+0 .0 2 mg/ L IBA+3%蔗糖 +0 .6 %琼脂 ;最适移栽基质为河沙∶珍珠岩 =2∶ 1。增殖周期为Tissue culture of Drgeon Begonia can use the young leaves as explants .The adventitions buds can occur from callus of initiated explants. The optimum medium for inducing adventitions buds from explants is MS with 1.0mg/L 6 BA+0.1mg/L NAA+3% sucrose+0.6% agar, for adventitions buds multiplication is MS with 0.8mg/L 6 BA+0.08mg/L NAA+3% sucrose and 0.6% agar, for rooting is 1/2 MS+0.08mg/L NAA+0.02mg/L IBA+3% sucrose and 0.6 % agar. The best matrix for transplanting plants is sand(2):perlite(1).The cycle of the adventitions buds multiplication is 35 days every time.

关 键 词：龙翅海棠 组织培养 快繁技术 幼嫩叶片 培养基 移栽基质 

分 类 号：S685.99[农业科学—观赏园艺]