三个猪品种CAST基因PCR-RPLP分析  

Analysis of CAST Gene in Three Breed by PCR-RFLP

作　　者：程丰[1] 郁枫[1] 王金勇[2] 白小青[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100 [2]重庆市养猪科学研究院,重庆402460

出　　处：《动物科学与动物医学》2004年第4期46-48,共3页Animal Science & Veterinary Medicine

基　　金：国家"十五"攻关项目:(任务书编号:2002BA514A-7-5);重庆市"十五"创新工程重大专项:(合同号:7847)

摘　　要：采用3种限制性内切核酸酶对杜洛克、内江猪和荣昌猪共153头的钙蛋白酶抑制蛋白(CAST)基因进行PCR-RFLP分析。结果表明:HinfI、MspI和RsaI均检测到多态性,同时还发现一个杜洛克个体的HinfI-RFLP出现120bp和80bp突变片段。χ2检验表明:HinfI、MspI和RsaI酶切产生基因已经达到Hardy-WeLnberg平衡状态(p<0.05);数量上三个品种的优势基因型ABCCEE、AACCEE、AACCFF,在99﹪程度上分别与相应的品种有关。Through PCR-RFLP ,the polymorphisms of CAST gene were analysised respectively by HinfI,MspI and RsaI in 153 pigs including three breeds:Drouc,Neijiang Pig and Rongchang Pig.The result revealed: there were polymorphisms respectively by HinfI,MspI and RsaI.An individual with a specific fragment about 120 bp and 80 bp and without a fragment about 350 bp was detected in Druco. The test ofχ2 suggested that all of alleles were in the state of Hardy-WeLnbergang equilibrium(p<0.05=.Each dominant genotype of ABCCEE?AACCEE?AACCFF in quantity was repectively associated at 99% degree of accuracy with 3 breeds: Drouc,Neijiang Pig and Rongchang Pig.

关 键 词：猪 CAST 基因多态性 PCR-RPLP分析 品种 钙蛋白酶抑制蛋白 肉质嫩度 

分 类 号：S828[农业科学—畜牧学]