不同年代铅锌矿渣中细菌的重金属和抗生素抗性特征  

Resistant Features of Heavy Metals and Antibiotics in Bacteria from Mine Tailings with DifferentDeposited History

作　　者：张汉波[1] 施雯[1] 于春蓓[1] 沙涛[1] 程立忠[1] 段昌群[2] 

机构地区：[1]云南大学生物系,云南昆明650091 [2]云南大学环境科学系,云南昆明650091

出　　处：《农业环境科学学报》2004年第2期348-351,共4页Journal of Agro-Environment Science

基　　金：云南省自然科学基金资助项目(2002C0001Q);云南省教育厅自然科学基金重点项目(02ZD013);云南省工业微生物发酵工程重点实验室开放基金项目(KF-2001-01)

摘　　要：利用主成分分析法,研究了不同年代铅锌矿渣堆化学性质的改变同细菌群体重金属和抗生素抗性特性的关系。结果表明,随堆积年代的延长,矿渣堆5～10cm和25～30cm层面的化学性质差异增加。与这种空间上的环境因子分化相对应,312株随机分离的细菌菌株和97株节杆菌菌株在5种重金属和4种抗生素的抗性比例上也发生了变化。堆积时间在80a左右的C矿渣抗性菌株比例同较年轻的矿渣A(约10a)、B(约20a)有较大差别,但有趣的是矿渣A和矿渣C在层面上的差异都较矿渣B大。The relationship between resistance of heavy metals and antibiotics in bacteria and the chemical features of three mine tailings, which were abandoned at approximately 10, 20 and 80 years ago, were investigated by principal component analysis (PCA). We have found that with increase of abandoned history, the difference of the components for layers 5～ 10 cm and 25～ 30 cm was enlarged. Corresponding to the environmental heterogeneity, the resistance of heavy metals and antibiotics from 312 bacterial strains and 97 strains of Arthrobacteria isolated randomly from mine tailings differentiated. Tailings C, with a deposited history of around 80 years, exhibited the greatest difference in terms of resistance of bacteria from tailings A and B. Interestingly, both of tailings C and tailings B displayed much more difference of resistance than that of tailings B, between the layer 5～ 10 cm and 25～ 30 cm.

关 键 词：铅 锌 矿渣 细菌 重金属 抗生素 抗性特征 主成分分析 重金属污染土壤 

分 类 号：S151.93[农业科学—土壤学]