MALDI-TOF质谱技术分析加州海兔口腔神经节中超微量Leu-Leu多肽酶  

Analysis of ultramicro Leu-leu endopeptid ase in buccal ganglion of Aplysia California by MALDI-TOF mass spectrometry.

作　　者：黄河清[1] 

机构地区：[1]厦门大学生命科学学院分析测试中心,厦门361005

出　　处：《分析仪器》2004年第2期23-27,共5页Analytical Instrumentation

基　　金：国家自然科学基金 ( No.40 2 70 63 3 );厦门市科技项目和海洋与环境教育部重点实验基金资助项目

摘　　要：利用丙酮 -水 -酸 (4 8∶ 5 0∶ 2 V/ V)萃取系统 ,从加州海兔 (Aplysia California,AC)口腔神经节中提取 L eu- L eu多肽酶 ,并用反相 HPL C进行纯化。选用具有三对 L eu- L eu结构的酸性多肽 (acidic peptide,AP)作为探针 ,以基质辅助激光解吸电离飞行时间 (MAL DI- TOF)质谱技术研究了海兔口腔神经节中的超微量 L eu- L eu多肽酶。对 L eu- L eu多肽酶的活性与类型及温度处理效果进行讨论。Leu-Leu endopeptidase in buccal ganglion (BG) of Aplysia California (AC) was extracted with acetone- water-HCl (48∶50∶2) system and purified by RP-HPLC. Using acidic peptide with three pairs of Leu-Leu structure as probe , the ultramicro Leu-Leu endopeptidase in BG of AC was analyzed by MALDI-TOF-MS. The activities and kinds of Leu-Leu endopeptidase and the effect of temperature treatment are discussed. The technique is also applicable for analyzing ultramicro endopeptidases in other tissues or cells.

关 键 词：质谱技术 海兔 酸性多肽 Leu-Leu多肽酶 超微量分析 口腔神经节 MALDI-TOF 

分 类 号：O657.63[理学—分析化学]