重组大豆KUNITZ型胰蛋白酶抑制剂Ti^d的大肠杆菌表达及抑制活性研究  

Expression and Activity Study of Soybean Kuntiz-type Trypsin Inhibitor

作　　者：丁波[1] 董爱武[1] 詹树萱[1] 忻骅[1] 曹凯鸣[1] 

机构地区：[1]复旦大学生命科学学院生物化学系,上海200433

出　　处：《复旦学报（自然科学版）》2004年第2期210-213,共4页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目 (30 170 0 96 )

摘　　要：利用大肠杆菌高效表达系统表达大豆Kunitz型胰蛋白酶抑制剂 (SBTi A2 )Tid,并利用亲和层析的方法纯化得到有抑制胰蛋白酶活性的SBTid.对重组SBTid的 pH稳定性和温度稳定性与天然SBTia进行了比较研究 .结果表明重组SBTid在高 pH下具有更好的稳定性 。One kind of Soybean kuntiz-type trypsin inhibitors(SBTi)-Ti^(d) was expressed in E.coli and purified by affinity chromatograph. The recombinant Ti^(d) showed trypsin inhibitory activity in vitro . The pH stability and thermal stability of Ti^(a) and recombinant Ti^(d) were studied. Compared with the natural SBTi^(a),SBTi^(d) exhibited a better stability in a high pH spectrum and a similar thermal stability at less than 50 ℃ .These data suggested that SBTi^(d) has a promising application potential as a bio-pesticide and insect-resistant gene in crop designing.

关 键 词：重组大豆胰蛋白酶抑制剂 稳定性 抗虫 

分 类 号：Q946.1[生物学—植物学] S435[农业科学—农业昆虫与害虫防治]