dst6,拟南芥中一个可能的新的det2等位基因突变株(英文)  

dst6, a Putative New Allele of det2 Mutant in Arabidopsis thaliana

作　　者：徐巧婷[1] 刘天磊[2] 钱堃[1] 胡秉桢[1] 陈人峰[1] 曹树青[1] 朱勇[1] 周红军[1] 蒯本科[1] 

机构地区：[1]复旦大学生命科学学院生物化学系,上海200433 [2]复旦大学生命科学学院遗传学研究所,上海200433

出　　处：《复旦学报（自然科学版）》2004年第2期214-219,共6页Journal of Fudan University：Natural Science

基　　金：ProjectsupportbyNationalNaturalScienceFoundation(39870 4 5 2 )

摘　　要：在黑暗条件下筛选拟南芥滞绿突变体时 ,得到一株突变体 ,dst6 ,表现出典型的油菜素突变体的特征 .遗传分析表明其是单基因隐性突变 .利用简单序列多态性标记 ,将其定位于 2号染色体的底部 .粗定位的结果和形态学特征表明dst6可能是一个det2等位基因突变株 .通过对dst6中DET2基因的测序分析 ,证明了dst6可能是一个新的det2等位基因突变株 .In an attempt to screen stay-green mutants in Arabidopsis in the absence of light,a mutant displaying the characteristics of brassinosteroids mutants was isolated. This mutant, dst6 ,carries a single recessive mutation. Using molecular genetic markers,the mutation was mapped to the bottom of chromosome Ⅱ,which is around the region of DET2,one of the genes known to cause brassinosteroids mutation. The analysis of cDNA and genomic sequence of DET2 in dst6 revealed a frame-shifting insertion. These results suggest that dst6 is a putative new allele of det2 .

关 键 词：拟南芥 dst6 det2 定位克隆 油菜素甾体 序列分析 

分 类 号：Q943[生物学—植物学]