蝴蝶兰快速经济有效繁殖技术的研究(英文)  

A Reliable Protocol for Plant Regeneration from Pedicel Axillary Bud of Phalaenopsis in vitro

作　　者：王敬文[1] 明凤[1] 叶鸣明[1] 董玉光[2] 梁斌[1] 陈龙英[2] 沈大棱[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,遗传工程国家重点实验室,生物多样性科学研究所,生物多样性与生态工程教育部重点实验室,上海200433 [2]上海市农业科学院引种中心,上海201106

出　　处：《复旦学报（自然科学版）》2004年第2期230-234,共5页Journal of Fudan University：Natural Science

基　　金：ProjectsupportedbytheShanghaiDevelopmentFoundation (0 13912 0 0 7)

摘　　要：以蝴蝶兰 (Phalaenopsis)花梗腋芽为外植体 ,研究出一种高效、简便的诱导原球茎 (Protocorm)的方法 .在不含其他有机添加物 ,只添加BA (3mg/L) +NAA (0 .1mg/L)的MS培养基中 ,原球茎诱导效率达 80 % .相同培养基继代 ,4周内即可发育成幼苗 .在含有IAA(0 .1mg/L)的MS培养基中 ,4 0 %的幼苗可以诱导生根 ,并移植成功 .由于此方法简便、经济有效 ,可应用于工厂化生产 ,而且获得的优质原球茎也为进一步的遗传改良提供材料 .An efficient and simple method of high frequency protocorm regeneration from pedicel axillary bud of Phalaenopsis is described. Pedicel axillary buds were cultured on Murashige and Skoog's basal medium (MS) with combinations of 6-benzylaminopurine (BA) (2-3 mg/L) and α -naphthaleneacetic acid (NAA) (0.1-0.5 mg/L). Medium supplemented with 3 mg/L BA in combination with 0.1 mg/LNAA produced the best response in protocorm occurrence(80%) without addition of any organic material. On subculture to the same medium, protocorm produced the shoots within 4 weeks of culture and well developing shoots were obtained separately. 40% of developed shoots produced root on medium supplemented with 0.1 mg/L IAA and the plants were transferred to moss for growth. Because of the simplicity for culture medium ingredient, it is a reliable protocol for plant regeneration of Phalaenopsis factory production in developing country and is also useful to genetic improvement programs.

关 键 词：蝴蝶兰 花梗腋芽 原球茎 

分 类 号：Q943.1[生物学—植物学]