苹果离体叶片培养直接体细胞胚胎发生的形态学研究  

MORPHOLOGICAL STUDY OF DIRECT SOMATIC EMBRYO-GENESESFROM IN VITRO LEAVES OF APPLE(Malus domestica Borkh.)

作　　者：达克东[1] 张松[1] 臧运祥[1] 吴禄平[2] 束怀瑞[1] 

机构地区：[1]山东农业大学园艺学院,山东泰安271018 [2]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《核农学报》2004年第2期118-120,共3页Journal of Nuclear Agricultural Sciences

基　　金：山东省"三零"工程资助项目

摘　　要：研究了苹果离体叶片直接体细胞胚胎发生体系和外植体直接体细胞胚胎发生过程中的形态学特征。结果显示 ,苹果直接体细胞胚胎发生过程经历原胚、球形胚、心形胚、子叶形胚、成熟胚等阶段 ;子叶形胚以前的阶段没有观察到形态学多样性 ,发育至子叶形胚期的直接体细胞胚胎表现出形态学多样性 ,分别观察到一子叶胚、两子叶胚、三子叶胚、杯状胚、多子叶胚、畸形胚等 ,这些类型的胚最终都能发育成正常的成熟胚进而发育成小植株。Direct somatic embryogenesis from in vitro leaves of apple cultivar Gala(Malus domestica Borkh.) was established.Morphological characters of the developing direct somatic embryo on the explant were studied.The results showed that apple direct somatic embryo undergo pre-embryo,ball-embryo,heart-embryo,cotyledon-embryo and mature-embryo to become plantlet.No polymorphism was observed in the developing stage before cotyledon-embryo,but embryos in cotyledon-embryo stage showed polymorphic characters,monocotyledon-embryo,dicotyledon-embryo,tricot-embryo,multicotyledon-embryo,cup-embryo,clavated-embryo,radial-embryo and calabashy-embryo were observed.All these kinds of embryos could develop into normal mature-embryo and become plantlet.

关 键 词：苹果 叶片 离体培养 直接体细胞胚胎 形态学 

分 类 号：S661.1[农业科学—果树学]