抗链霉素单克隆抗体的制备和鉴定  

PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES AGAINST STREPTOMYCIN

作　　者：王谦[1] 齐孟文[1] 何方洋[1] 杨根海[1] 曲勍[1] 

机构地区：[1]中国农业大学,北京100094

出　　处：《核农学报》2004年第2期158-160,157,共4页Journal of Nuclear Agricultural Sciences

摘　　要：用CDI法合成链霉素全抗原 ,免疫BALB c小鼠 ,采用杂交瘤技术得到了一株能稳定分泌抗链霉素单克隆抗体的杂交瘤细胞。经ELISA鉴定 ,SM McAb为IgG1类 ;细胞培养上清的效价达 1 0 4以上 ,腹水的达 1 0 8以上 ;与双氢链霉素交叉反应率为 2 0 0 % ,与庆大霉素、卡那霉素无交叉反应。The antigen of streptomycin synthesized by CDI reaction was used for preparation of a hybridoma cell line which secretes monoclonal antibodies against streptomycin.The antibody was characterized by IgG 1 isotype, the titers of antibody in cell supernatant and ascites were 1∶10 4 and 1∶10 8, respectively. The antibody against streptomycin was selective toward dihydrostreptomycin with cross-reactivity of about 200%,and.wasn't detective cross-reactivity toward gentamicin and kalamycin.

关 键 词：链霉素 单克隆抗体 制备 鉴定 杂交瘤技术 抗生素 酶联免疫吸附分析 药物残留 饲料添加剂 

分 类 号：S852.5[农业科学—基础兽医学]