RFP-BSA免疫家兔及RFP特异IgG的检测  

RABBIT IMMUNIZATION WITH RFP-BSA CONJUGATE AND RFP SPECIFIC ANTIBODY (IgG) DETECTION

作　　者：李林丰[1] 王晶[1] 李世荫[1] 赵翀 

机构地区：[1]北京医科大学第三医院皮肤科,北京100083 [2]北京医科大学第三医院免疫教研组,北京100083

出　　处：《中国抗生素杂志》1992年第5期356-358,共3页Chinese Journal of Antibiotics

摘　　要：用利福平(RFP)与牛血清白蛋白(BSA)在碱性条件下反应生成稳定的偶联物。偶联比约为RFP:BSA=3.1:1。用此偶联物免疫家兔获得抗血清经ELISA竞争抑制实验证明了RFP特异IgG的存在。其效价约为1:4000。说明RFP具有免疫原性,在一定条件下可以使动物致敏。初步建立了田ELISA检测RFP特异IgG的方法。Stable Rifampicin (RFP) and bovine serum albumin (BSA) conjugate was prepared under the alkaline condition.The conjugat- ion ratio of RFP to BSA is about 3:1. Rabbits were succesfully immunized with these conjugates and the specific IgG to RFP in these rabbits was detected and identified by indirected ELISA and competitive ELISA. The titre is about 1:4000.Thus, the immunogenicity of RFP conjugate to rabbit is demonstrated and the detection of RFP spicific antibody in RFP allergy is become possible.

关 键 词：特异IgG ELISA 利福平 

分 类 号：R978.1[医药卫生—药品]