秋水仙碱诱导白杨三倍体新途径的发现  

Discovery of a new way of poplar triploids induced with colchicine after pollination.

作　　者：康向阳[1] 张平冬[1] 高鹏[1] 赵芳[1] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点开放实验室

出　　处：《北京林业大学学报》2004年第1期1-4,共4页Journal of Beijing Forestry University

基　　金：国家林业局重点项目 ( 1 999 1 2 )的部分内容

摘　　要：以银腺杨等为母本 ,毛白杨为父本 ,授粉后一定时间采用秋水仙碱溶液处理花序 ,共获得 40 2粒种子 ,成苗10 7株 ,从中检出 2 1株银腺杨×毛白杨三倍体 .证明授粉后 2 4～ 3 6h是施加秋水仙碱诱导白杨三倍体的有效时期 ,三倍体得率最高达 57 1% .通过这一途径获得的白杨三倍体同样具有十分明显的生长优势 ,2年生实生苗的平均苗高、地径超出全部后代群体总平均值的 2 9%和 65% ,其中以BY3 2 0等表现最佳 ,分别超出群体平均数的 55%和119% .Populus alba×P. glandulosa)×P. tomentosa triploids were found from the 107 seedlings,which were obtained from 402 hybrids between P. alba×P. glandulosa etc. (male parent) and P. tomentosa(female parent) by treating the pollinated flowers with colchicine. It indicated that the effective period of inducing triploids with colchicine was 24～36 h after pollination. The highest ratio of obtained triploids reached 57 1%. The growth of triploids also showed obvious superiority over normal diploids. The average stem height and basal diameter of 2 year old triploid seedlings was 29% and 65% larger than the average growth of all the offspring respectively. The growth of BY320 is the best,of which stem height and basal diameter was 55% and 119% higher than the average growth respectively. A new way for inducing poplar triploid was obtained through the present research.

关 键 词：白杨 授粉 秋水仙碱 三倍体 

分 类 号：S792.119[农业科学—林木遗传育种]