知母活性成分ZDY101对脑内定位注射鹅膏蕈氨酸拟痴呆大鼠的影响  

Effects of ZDY101, an active component from Zhimu, on rat dementia model produced by intracranial injection of ibotenic acid

作　　者：孙启祥[1] 胡雅儿[1] 管宏[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室,上海200025

出　　处：《核技术》2004年第4期297-300,共4页Nuclear Techniques

摘　　要：观察中药知母活性成分ZDY101对大鼠脑内注射兴奋性氨基酸所致拟痴呆模型的治疗作用。将IBO2μg/0.5μL双侧缓慢注入SD大鼠脑基底核部,造成局部神经元变性后产生拟似痴呆症状。ZDY101分高、中、低三个剂量组连续灌胃给药90d,以避暗试验评价动物学习记忆能力,3H-QNB单点结合法测定脑组织M受体密度。大鼠脑内注入IBO后,学习、记忆功能明显下降,脑M受体密度显著降低。给予ZDY101后,学习、记忆功能及脑M受体密度均有明显提高,且二者具有量效相关性。表明ZDY101很可能通过上调脑M受体密度而改善脑功能,是一种值得深入研究的抗老年痴呆症药物。A dementia model was produced in adult Sprague-Dawley rats by bilateral injection of ibotenic acid, 2μg in 0.5μL per side, into basal ganlion. ZDY101, 3.6, 18, 90 mg·kg? ·d? , was given through gastric tube to 3 groups 1 1 of the model rats for 90 days. The learning and memory ability of model rats without treatment declined compared with sham-operated controls, the composite index (number of wrong reponse in 5 min divided by latent period) being 0.71±0.30 vs 0.10±0.13 respectively. The brain M receptor density also decreased as compared with sham-operated controls, being 891±150 vs 1039±214 fmol·mg? protein respectively. ZDY101 simultaneously up-regulated the 1 brain M receptor density and improved the memory and learning ability of model rats. For the middle dose group, the M receptor density was 1028±145 fmol·mg? protein (P<0.05) and the composite index of step-through test was 1 0.20±0.18 (P<0.01). Since there was a significant correlation between M-receptor density and result of step-through test, it is apparent that the mechanism of action of ZDY101 is quite different from that of cholinesterase inhibitor. It is highly probable that the improvement of memory was due to the up-regulation of brain M receptor density

关 键 词：ZDY101 脑M受体 老年痴呆症 

分 类 号：R81[医药卫生—放射医学] R817[医药卫生—临床医学]