应用改良浸种法将外源DNA导入大豆的遗传育种效应研究  

Genetic and Breeding Effects of Introduction of Exogenous DNA into Soybeans by Improved Seed-Soaking Method

作　　者：江巨鳌[1] 麻浩[2] 周治淼[3] 田森林[1] 黎克衡 

机构地区：[1]湖南农业大学校级实验室管理中心,湖南长沙410128 [2]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [3]湖南农业大学教务处,湖南长沙410128 [4]张家界市永定区农业局国营农场,湖南张家界427000

出　　处：《湖南农业科学》2004年第2期10-13,共4页Hunan Agricultural Sciences

基　　金：湖南省教育厅资助项目(95-12)

摘　　要：应用"改良浸种法"将玉米、西洋参DNA导入大豆,结果表明,该导入方法可降低导入当代种子发芽率、出苗率,导入后代在生育期、株高、单株结荚数、百粒质量、节数、种脐色、蛋白质和异黄酮含量等性状上产生广泛变异,且许多变异性状(如早熟性、株高)在早世代就能迅速稳定遗传。导入引起的变异频率在D2代为7 6%～18 9%,D3代为21 5%～26 4%,随世代的增加变异频率明显增加。并进一步探讨了这些性状的变异特点。同时,为了提高育种效率,提出了导入后代的选择方法。The improved seed-soaking method was used to introduce the exogenous DNA of maize and America Ginseng (Panax quinquefolium) into soybean and the biological, genetica and breeding effects of the introduction in this experiment were discussed. The results indicated that the method could not only reduce the germinating and seedling percentage of the soaking seeds and effect the plant's development, but also result in the heritable variations of many agronomic characteristics. The variation characteristics included growth period, plant height, pod number per plant, seed weight per plant, 100-seed weight, node number, hilum color, protein content and isoflavone content etc. Many variable traits, such as early-maturity and short-height, could rapidly and stably inherit and could be used as materials for breeding selection. The variation frequency of introducing was 7.6～18.9% in D2,and 21.5～26.4% in D3. The frequency seemed to rise with the increase of the generations. The variation characteristics of the traits were further explored and the selection methods of progeny with exogenous DNA were brought out for improving the breeding efficiency.

关 键 词：品种改良 浸种法 外源DNA 基因导入 大豆 遗传育种 

分 类 号：S565.1[农业科学—作物学]