RNase P核酶对人巨细胞病毒UL54基因mRNA体外切割作用  

Targeted in vitro Cleavage of Human Cytomegalovirus UL54 mRNA Segments by RNase P

作　　者：周乐平[1] 李弘剑[1] 陈浩军[1] 李月琴[1] 何华坤[1] 王波[1] 周天鸿[1] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632

出　　处：《中国病毒学》2004年第2期110-113,共4页Virologica Sinica

基　　金：国家自然科学基金(30370776);广东省自然科学基金重大项目(36703);广东省自然科学基金(021162;000718)

摘　　要：外部引导序列(EGSs)是mRNA靶序列互补并引导RNase P切割的小RNA片段。我们设计与人巨细胞病毒HCMV(Human Cytomegalovirus) UL54基因mRNA序列互补的EGSs,将其与大肠杆菌来源RNase P催化核心M1 RNA构建成M1GS核酶。通过对UL54基因亚克隆片转录产物体外切割研究,证实该核酶具备对UL54 mRNA片段的特异切割能力,可以发展成为一种抗病毒试剂。External guide sequences (EGSs) are oligonucleotides that consist of a sequence com- plementary to a target RNA and specific cleavage occurs at such locations acted by M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli. A sequence-specific ribozymes (M1GS-C6 RNA) derived from EGSs and M1 RNA were used to target a region of UL54 mRNA encoding major DNA polymerase of Human Cytomegalovirus in vitro. The results showed that M1GS ribozyme can function as an antiviral agent and effectively cleave UL54 mRNA.

关 键 词：人巨细胞病毒HCMV UL54基因 MRNA RNaseP 外部引导序列 

分 类 号：R346[医药卫生—基础医学]