转RGSV-SP基因水稻植株的再生  

Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

作　　者：金凤媚[1] 林丽明[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建福州350002

出　　处：《中国病毒学》2004年第2期146-148,共3页Virologica Sinica

基　　金：国家自然科学基金资助项目(39970486);福建省自然科学基金资助项目(0010011);福建省青年科技人才创新基金资助(2002J028)

摘　　要：通过农杆菌介导将水稻草矮病毒(Rice grassy stunt virus, RGSV)编码病害特异蛋白基因sp导入台农67及中花6号品种。经过筛选,再生,获得转化植株。PCR及Southern点杂交分析结果初步表明目的基因片段整合到水稻基因组中。RT-PCR Southern点杂交结果表明目的片段已在植株体内进行了转录。Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.

关 键 词：农杆菌 水稻草矮病毒 RGSV SP 台农67 中花6号 品种 水稻 基因片段 

分 类 号：S511[农业科学—作物学]