利用酰胺型手性固定相高效液相色谱法对格列美脲顺反异构体的分离与测定  

Direct Separation and Quantitative Determination of Transglimepiride by High Performance Liquid Chromatography Using an Amide Type Chiral Stationary Phase

作　　者：宋雅茹[1] 王德发[2] 牛丽萍[1] 胡玉萍[1] 杨亦平[1] 侯冬岩[3] 

机构地区：[1]北京师范大学化学系,北京100875 [2]国家标准物质研究中心,北京100013 [3]鞍山师范大学化学系,鞍山114005

出　　处：《分析化学》2004年第4期435-438,共4页Chinese Journal of Analytical Chemistry

摘　　要：利用高效液相色谱法在一种酰胺型手性固定相上直接分离了格列美脲顺反异构体。讨论了流动相组成、柱温度和流速等色谱条件对分离的影响。优化的实验条件为 :流动相组成正己烷 :1 ,2 二氯乙烷∶甲醇(88∶8∶4 ,V/V/V) ;柱温 :2 5℃ ;流速 :1 .8mL/min。得到的分离度和选择性因子分别为 1 .86和 1 .1 2。在 2 88～ 32 0mg/L的浓度范围内建立了反式格列美脲的标准曲线 ,线性系数为 0 .99998;检出限为 0 .4 7mg/L。方法简单 ,可靠 。Trans- and cis-glimepiride were directly separated by a new established high performance chromatographic method, which involved the use of Chirex 3005 column. Several parameters were studied. Simple and reliable separation was achieved, with the separation factor (alpha) 1.12 and the resolution factor (R-s) 1. 86, when using the optimized mobile phase composed of n-hexane: 1,2-dicholoethane: methanol (88/8/4 by vol) at 25degreesC and 1.8 mL/min. Standard linear calibration curve was established for trans-glimepiride, over the range of 3 similar to 320 mg/L, with the correlation coefficient of 1. 0000. The minimum limit of detection was 0. 47 mg/L. Precision of the assay was gratifying and the method has been used to monitor and identify quantitatively the products of glimepiride.

关 键 词：格列美脲 顺反异构体 分离 测定 酰胺型手性固定相 高效液相色谱法 降糖药 含量测定 

分 类 号：O657.7[理学—分析化学]