鲁米诺-过硫酸钠化学发光体系测定硝苯地平  

Flow Injection Chemiluminescence Method for Determination of Nifedipine Based on Persulfate-Luminol Reaction

作　　者：何树华[1] 吕弋[1] 何德勇[1] 胡玉斐[1] 章竹君[1] 

机构地区：[1]西南师范大学化学化工学院分析科学研究所

出　　处：《分析化学》2004年第4期474-476,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 0 175 0 3 9)

摘　　要：过硫酸钠在碱性条件下能氧化鲁米诺产生微弱化学发光 ,硝苯地平能大大增强此发光。基于此 ,建立起了一种直接测定硝苯地平的流动注射化学发光方法。该方法线性范围为 0 .0 5～ 5 .0mg/L ;检出限为0 0 1 7mg/L ;相对标准偏差为 2 .8% (硝苯地平 0 .5mg/L ,n =1 1 )。利用该方法测定了硝苯地平片剂含量 。A novel chemiluminescence (CL) system coupled with flow injection technique was developed for determination of nifedipine. It was found that the luminol could react with persulfate in sodium hydroxide media, producing a weak chemiluminescence, which could be sensitized intensively by nifedipine. The sensitized chemiluminescence intensity was a linear function of nifedipine concentration. Under the optimal conditions (luminol concentration: 1.0 x 10(-4) mol/L, persulfate concentration: 1.0 x 10(-3) mol/L, sodium hydroxide concentration: 0.2 mol/L), the proposed method had a linear range of 0.05 similar to 5.0 mg/L with a regression equation DeltaI = 330.2C + 52.83 (C: nifedipine concentration, mg/L, r = 0.9991). The detection limit was 0.017 mg/L with a RSD of 2.8% (nifedipine concentration: 0.5 mg/L, n = 11). This method was sensitive, simple, rapid, and was successfully applied to the determination of nifedipine in tablets.

关 键 词：硝苯地平 心血管系统药物 片剂 含量测定 鲁米诺-过硫酸钠化学发光体系 流动注射法 

分 类 号：TQ463[化学工程—制药化工] O657.3[理学—分析化学]