阿维链霉菌bkdF的基因中断对阿维菌素合成的影响  

Effects of bkdF Interruption on Avermectin Biosynthesis

作　　者：朱浩君[1] 梁运祥[1] 周俊初[1] 郑应华[2] 

机构地区：[1]华中农业大学农业微生物重点实验室,武汉430070 [2]北京生物医药研究所,北京100091

出　　处：《微生物学报》2004年第2期194-197,共4页Acta Microbiologica Sinica

摘　　要：以阿维菌素B组分菌株StreptomycesavermitilisBjbm0 0 0 6为出发菌株 ,用PCR方法构建支链α 酮酸脱氢酶基因bkdF(Branched chainα ketoaciddehydrogenasegene)的重组质粒pHJ5 816 (pHZ135 8 bkdF&Ermr)对其进行基因中断 ,得到重组菌株Bjbm5 816。经HPLC检测和核磁共振分析发现 ,Bjbm5 816发酵产物产生的单一组分新化合物为Oligo mycinA。Bjbm0006 was a strain which produces four avermectin B component. bkdF (branched-chain α-keto acid dehydrogenase gene) gene involved in the biosynthesis of avermectin B was cloned by PCR from Bjbm0006 chromosome, and inserted with a Erm r gene to construct a recombinant plasmids pHJ5816 (pHZ1358∷bkdF&Erm r). The bkdF-interrupted mutant Bjbm5816 was received through homologous double crossover between the pHJ5816 and the chromosome. The interruptant Bjbm5816 produce single novel compound and it was attested to be Oligomycin A by MS, 1H-NMR, 13 C-NMR and two-dimension NMR.

关 键 词：阿维链霉菌 阿维菌素 基因中断 支链a-酮酸脱氢酶基因 菌株 生物合成 

分 类 号：Q78[生物学—分子生物学]