细菌除草剂黄单胞菌反枝苋致病菌的筛选  

Screening of Bacterial Herbicide Strain Xanthomonas campestris pv. retroflexus from Rhizosphere

作　　者：李明智[1] 李永泉[1] 徐凌[1] 庄晓峰[1] 孙自玲[1] 

机构地区：[1]浙江大学生命科学学院,杭州310027

出　　处：《微生物学报》2004年第2期226-229,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 3 70 93 9)~~

摘　　要：从杂草反枝苋根际土壤中分离到大量的根际细菌 ,利用谷氨酰胺合成酶抑制剂模型和蛋白核小球藻筛选模型进行快速、高效的初筛 ,并结合温室盆栽复筛 ,筛选出一株具有较强除草活性的细菌 野油菜黄单胞菌反枝苋致病变种。温室盆栽试验表明 ,该黄单胞菌对反枝苋、荠菜等双子叶杂草具有较强的抑制作用。Xanthomonas campestris pv. retroflexus with herbicidal activity was screened from rhizosphere of redroot pigweed (Amaranthus retroflexus L.) using the glutamine synthetase inhibitor and the microscreen of Chlorella pyrenoidosa model. Petri dish bioassays of model plants and weeds were used to evaluate the influence of Xanthomonas campestris pv. retroflexus on growth of the selected weeds. Broad leaf weeds belonging to dicots were confirmed to be its target weeds, such as Redroot pigweed and Shepherd's purse.

关 键 词：杂草 反枝苋 根际土壤 细菌除草剂 黄单胞菌 致病菌 蛋白核小球藻筛选模型 酶抑制剂模型 

分 类 号：S182[农业科学—农业基础科学] Q93-331[生物学—微生物学]