NCPP抗逆转录病毒鼠白血病病毒作用的研究  

Study on Anti -MuLV Effects of NCPP

作　　者：高尚先[1] 厉保秋[2] 曲守方[1] 康国华[1] 李守悌[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]山东大学毒理学研究所,济南250012

出　　处：《药物分析杂志》2004年第2期193-197,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用逆转录病毒鼠白血病病毒感染小鼠模型,研究无细胞短棒状杆菌纳米级制剂(NCPP,Nano-scale Productfrom Corynebacterium parvum)体内抗逆转录病毒鼠白血病病毒作用,初步评价其抗HIV的作用。方法:将逆转录病毒鼠白血病病毒(SRS8)T淋巴细胞株复苏后,加新鲜10%小牛血清的RPMI 1640培养液进行培养。每只小鼠腹腔注射逆转录病毒鼠白血病病毒(SRS8)T淋巴细胞1×105/0.3 mL。动物腹腔攻毒4 h后,NCPP给药组分高(2.0 mg·次-1·只-1)、中(0.5 mg·次-1·只-1)、低(0.125 mg·次-1·只-1)3个剂量组,肌肉注射,隔日1次,共5次;阳性对照组用双汰芝(200 mg·kg-1·d-1)灌胃,连续给药14 d。部分动物14 d后检测血常规,解剖取脾,计算脾指数。其余动物观察存活率。结果:小鼠感染病毒给药14 d时,阳性对照组及NCPP中剂量组体重较模型对照组有明显好转(P<0.05)。血液学指标变化明显,阳性对照组及NCPP中剂量组白细胞计数明显高于模型对照组(P<0.01),与正常对照组相比无明显差异。NCPP中剂量组脾指数明显高于模型对照组及正常对照组(P<0.01)。30 d存活率:NCPP高剂量组/中剂量组明显高于模型对照组(P<0.05)。60 d存活率:中剂量组,高剂量组/阳性对照组明显高于模型对照组(P<0.01,p<0.05)。结论:NCPP具有明显的抗逆转录病毒鼠白血病病毒作用,并能明显延长感染小鼠的生存期,提示具有一定的抗HIV作用。Objective: To choose mice model infected by MuLV, investigate the in vivo anti - MuLV effects of NCPP and pilot evaluate its possible effects for anti - HIV. Methods:T lymphocyte transfected with MuLV(SRS8) ,were cultured with the RPMI 1640 medium added to 10% calf serum. Cells(1×105/0. 3 mL) were injected into the peritoneal cavity of mice. NCPP was divided into 3 groups, high - dose group ( 2. 0 mg per mouse)、mean - dose group (0. 5 mg per mouse) and low - dose group(0.125 mg per mouse). After 4 hours,every dose group of NCPP was given intramascular injection every other day thereafter for totally 5 times; positive control group, combivir (AZT/ 3TC) ,was given by intragastrical administration with 200 mg·kg-1·day-1 for consecutive 14 days. 14 days after the injections of NCPP and combivir( AZT/3TC ) , some mice were weighed and measured the hematology index and splenic index after killed. The others,the survival rate was figured out. Results:The 14th day after given medicine, the weight change of positive control group and mean - dose group are better than model group ( P < 0. 05 ). The he matology index showed significant changes in both positive control group(combivir)and NCPP mean -dose group. The count of WBC was significant higher than that of model control group (P <0.01) and was not significant different with that of control group. NCPP mean - dose group induced significant higher splenic index than model control group and control group(P<0.01). Survival rate after 30 days: NCPP High - dose/mean - dose group were significant higher than that of model control group (P<0.05 ). Survival rate after 60 days: NCPP mean - dose group and High - dose/ positive control group were significant higher than that of model control group(P<0.01 ,P <0. 05 ) . Conclusions: NCPP showed the significant effects in and - MuLV and significantly prolonged the survival lives of infected mice. It supposed that NCPP had the and - HIV effects in some degree.

关 键 词：机会性感染 艾滋病 人类免疫缺陷病毒 NCPP 免疫功能 T淋巴细胞 逆转录病毒鼠白血病病毒 免疫调节剂 巨噬细胞 

分 类 号：R978.7[医药卫生—药品]