一种筛选稗草生防潜力菌的简易生测方法  

A Simple Bioassay Method for Screening Pathogenic Fungi to Weeds

作　　者：黄世文[1] 余柳青[1] 段桂芳[1] 李迪[1] 赵航[1] 乔凤云[1] 喻锦秀[2] 罗宽[2] 

机构地区：[1]中国水稻研究所,杭州310006 [2]湖南农业大学,长沙410128

出　　处：《中国生物防治》2004年第1期53-56,共4页Chinese Journal of Biological Control

基　　金：国家自然科学基金 (30 0 70 5 0 5 ) ;浙江省重点资助项目 (0 1110 2 4 6 9)

摘　　要：将催芽至露白的稗草和水稻种子直接接种在于PDA培养基上培养形成的 4种稗草病原菌 (交链孢菌、弯孢菌、禾长蠕孢菌稗草专化型、尖角突脐孢菌 )菌落上 ,观察稻、稗苗感病情况 ,调查根、芽生长抑制率。结果表明 ,4种菌对稻、稗的致病性和生长抑制率与常规生测法结果基本吻合。说明在培养基中初步测定、筛选稗草生防潜力菌是可行的。该方法与常规生测法比较 ,可节省一半时间左右 ,同时可减少工作量。Alternaria alternata, Curvularia lunata, Helminthosporium gramineum Rabenh f. sp. echinochloae and Exserohilum monoceras are pathogenic fungi of barnyardgrass (Echinochloa crus-galli). Germinated seeds (coleoptile and radicle just emerged) of barnyardgrass and rice were directly inoculated on colonies of the pathogenic fungi cultured on PDA plates. Infection and growth inhibition of barnyardgrass and rice seedlings were investigated after culturing for several days. Results showed that the pathogenicity and growth inhibition of barnyardgrass by the four pathogens were almost the same with the results of normal test. Comparing to the normal test, the bioassay can save half time and reduce workload for testing and screening.

关 键 词：稗草 PDA培养基 水稻种子 接种 菌落 病原菌 致病性 生长抑制率 生物测定法 

分 类 号：S451[农业科学—植物保护] S476