空肠弯曲菌67KD 外膜蛋白诱导抗核抗体生成  

Role of 67KD outer membrane protein of Campylobacter jejuni in inducing production of antinuclear autoantibodies

作　　者：夏文浪[1] 马宝骊[1] 

机构地区：[1]上海市免疫学研究所基础免疫研究一室上海第二医科大学免疫学教研室,上海200025

出　　处：《中国免疫学杂志》1992年第1期19-24,共6页Chinese Journal of Immunology

摘　　要：在分离提取空肠弯曲菌 S—131主要膜抗原成份67KD 鞭毛蛋白的基础上,用此蛋白成份经腹腔免疫8周龄雌性 KM 小鼠,每周一次,共12周,ELISA 方法动态测定小鼠血清中自身抗核抗体(包括抗 ds-DNA,ss-DNA 和组蛋白抗体)的水平。发现输注剂量为120—200μg/次/小鼠时能成功诱导上述各类自身抗体的产生,其中抗组蛋白抗体的阳性反应性最大,出现时间较早.本研究为从分子水平研究慢性感染诱致自身免疫的机理提供了新的实验依据,为抗组蛋白抗体作临床早期诊断的潜在作用提供了佐证.The KM mice(8=week-old,female)were immunized,i.p.,once a week,with outer membrane protein of ampylobacter jejuni S131.The mice were killed 12 weeks after the first injection.Serumof each mouse was collected or detecting of autoantibodies against double strained DNA,singlestrained DNA and total histones by ELISA ethod.These autoantibodies detected were inducedsuccessfully when the dose was 120-00μg/time/mouse.There was a good relation between dose andeffect.So our experimental odel provided an useful method and a novel pathway to explore theetiology and mmunological mechanisms of autoimmune diseases on molecular level.In addition,among hese three kinds of autoantibodies,the level of anti-histone antibodies was the highest and hetime it went up was the earliest,this indicated the potential significance for clinic in arlier iagnosisof some autoimmune diseases.

关 键 词：自身免疫 空肠弯曲菌 外膜蛋白 

分 类 号：R392.1[医药卫生—免疫学]