超重症联合免疫缺陷小鼠细胞免疫功能特征研究  

Cellular immunologic characteristics of super severe combined immunodeficient mice

作　　者：陈桦[1] 马宝骊[1] 畅波 李彦 汪歌 

机构地区：[1]上海市免疫学研究所基础免疫研究一室上海第二医科大学免疫学教研室,上海200025 [2]中国医学科学院医学实验动物研究所

出　　处：《中国免疫学杂志》1992年第2期82-86,共5页Chinese Journal of Immunology

摘　　要：超重症联合免疫缺陷小鼠(B.C.B-17scid-beige)是将自然杀伤细胞(NK)缺陷基因(bg 基因)导入重症联合免疫缺陷病小鼠(C.B-17scid)体内得到的一种新品系小鼠.为确定该鼠基本细胞免疫特征,我们测定了该鼠 T、NK 和淋巴因子激活的杀伤细胞(LAK)等的功能,以及聚肌胞(poly I-C)对该鼠 NK 细胞活性的影响。该小鼠脾淋巴细胞不能针对 T 细胞有丝分裂原刀豆蛋白(ConA)的刺激而产生增殖反应。同正常对照小鼠相差非常显著(P<0.01)。该鼠 NK 细胞活性(对 YAC-1细胞杀伤活性)明显低于亲代 scid 小鼠和正常小鼠(P<0.01),经聚肌胞(100μg/只)预先刺激后该鼠 NK 细胞活性未见增加.而亲代 scid 小鼠则有明显增加。该鼠脾脏细胞经白细胞介素—2刺激后,LAK 细胞活性(对 P815细胞杀伤活性)明显低于亲代 scid 小鼠和正常小鼠(P<0.01)。上述结果表明 B、C、B-17scid-beige 小鼠是一种 T、NK和 LAK 细胞联合免疫缺陷小鼠,该鼠 B 细胞功能也是缺陷的(详见它文)。上述缺陷是 scid 基因和 bg 基因共同作用的结果,该动物为建立人—免疫缺陷动物模型提供了一种更为理想的受体小鼠,并为基础免疫学研究提供了一种新的研究工具。The super severe combined immunodeficient mice(superscid)is a newly established mouse strain which was constructed by introducing the bg gene into C.B-17 scid mice.To identify its cellular immunologic haracteristics,we examined the T、natural killer(NK)and lymphokine-activated killer (LAK)cell activity in this mouse strain.The NK cell activity was studied by measuring the cytotoxicity to NK sensitive cell(YAC-1)and the influence of poly Ⅰ-C on it.The LAK cell activity was examined by measuring the cytotoxicity aganist NK resistant cell(p815). The spleen cells of B.C.B-17 scid-beige mice lacked responsiveness to a T cell mitogen (concanavalin A 5μg/ml,600.004±207.84cpm,266.67±29.84cpm for scid mice,p<0.05: vs 5957.33±825.07 for BALB/c mice,p<0.01);displayed a very low natural killer activity (7.41±5.3 at 100:1 ffector/target ratio,vs 73.2±17.7 for C.B-17 scid mice;37.6±11.7 for BALB/c mice P 0.01),which could not be enhanced by an interferon inducer(poly Ⅰ-C) (At 50:1 effector/target ratio,sham-treated mice 0:vs treated mice 0.2±0.7 P>0.05);showed a low LAK cell activity(6.40±6.9 for B.C.B-17 Scid-beige mice,vs 72.3±14.1% for C.B- 17 scid mice at IL-2 250u/ml;E/T 00:1,3 days,P<0.01).All these results implied that B. C.B-17 scid-beige mice are mmunodeficient in T、B、NK and LAK cell.The date showing B cell deficiency of this strain will be reported in another paper. The superscid mouse strain should be a more perfect animal model for establishing a Human/ immune-deficient(HID)model,which can overcome the undesirable cytotoxic activity in the C.B-17 scid mice.They also provide a new tool for basic immunological research.

关 键 词：T细胞 免疫缺陷 Scid-hu模型 

分 类 号：R392.12[医药卫生—免疫学]