高效液相色谱-质谱法鉴定总合草苔虫中的草苔虫内酯成分  

Identification of Bryostatins in Bugula neritina Extracts by High Performance Liquid Chromatography and Q-Tof Mass Spectrometry

作　　者：孙鹏[1] 李玲[1] 易杨华[1] 张诗龙[1] 周大铮[1] 张淑瑜[1] 

机构地区：[1]第二军医大学药学院海洋药物研究室,上海200433

出　　处：《色谱》2004年第2期144-146,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(29672050).

摘　　要：采用高效液相色谱分离与质谱检测的联用技术,对总合草苔虫中的草苔虫内酯成分进行定性分析。在以体积比为80∶20的甲醇 水溶液为流动相、采用ODS柱进行分离的色谱条件下,10种草苔虫内酯成分均可获得良好的分离。分析结果表明:深圳大亚湾产地的总合草苔虫中共含有9种已知和1种未知的草苔虫内酯成分。已知成分分别是bryostatin4,5,6(9),7,8,10,16,17,18,其中bryostatin7和17是首次从中国海域总合草苔虫中发现的2种痕量草苔虫内酯。该方法简便、准确、灵敏度高,可以很好的对总合草苔虫原药材及提取物中的草苔虫内酯成分进行定性分析。A method with tandem high performance liquid chromatography (HPLC) and Q-Tof mass spectrometry (MS) has been established for the qualitative assay of bryostatins in Bugula neritina extracts. Ten bryostatins were well separated on a Polaris C_(18)-5 column (4.6 mm i.d.×200 mm, 5 μm) with methanol and water (80∶20, v/v) as mobile phase. The bryostatins were qualitatively detected with the mass spectrometric detection. Ten bryostatins were found in the Bugula neritina collected from Gulf of Dayawan (Shenzhen, China). Mass spectra of the peaks led to the identification of nine known bryostatins that were bryostatins 4, 5, 6(9), 7, 8, 10, 16, 17, 18. Bryostatins 4, 5, 8, 10 could be further verified by the retention times of the corresponding standards. The two trace constituents, bryostatin 7 and 17, were for the first time found in (Bugula) neritina inhabiting in China sea. It is interesting that the result indicated the existance of a possibly new bryostatin in the mixture. The work above provides a quick and (accurate) assay method for the qualitative identification of the bryostatins in Bugula neritina (extracts.)

关 键 词：总合草苔虫 草苔虫内酯 成分分析 高效液相色谱 质谱法 

分 类 号：Q54[生物学—生物化学]