土壤中莠去津及其降解产物的提取及高效液相色谱-质谱分析  

Extraction of Atrazine and Its Degradation Products in Soil and Determination by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：任晋[1] 蒋可[1] 徐晓白[1] 

机构地区：[1]中国科学院生态环境研究中心

出　　处：《色谱》2004年第2期147-150,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(批准号:29977032).

摘　　要：建立了高效液相色谱 质谱联用(HPLC MS)选择离子检测(SIM)分析环境土样中的痕量莠去津及其降解产物脱乙基莠去津(deethylatrazine,DEA)、脱异丙基莠去津(deisopropylatrazine,DIA)、羟基化莠去津(HA)的方法。土样用双蒸水超声提取,然后用WatersOasisMCX固相萃取小柱富集纯化土样提取液,测得莠去津及其降解物在不同加标浓度(4 5～120ng/g)下的回收率为:莠去津40 4%～82 0%,DEA60 6%～86 5%,DIA69 2%～86 1%,HA30 1%～80 3%。应用源内离子碰撞诱导解离技术能够观察母体和其特征碎片离子的情况,判断被分析物是否存在,增加了实验结果的可靠性。对某农药厂附近农田的表层土中的莠去津和其降解产物进行定性分析,验证了环境土样中痕量降解产物的存在。Atrazine and its degradation products such as deethylatrazine (DEA), deisopropyl-(atrazine) (DIA) and hydroxyatrazine (HA) in soil samples were extracted by Waters Oasis MCX cartridges, then determined by high performance liquid chromatography-atmospheric pressure chemical ionization-collision induced dissociation-mass spectrometry (HPLC-APCI-CID-MS). The recoveries of atrazine, DEA, DIA and HA fortified in the range of 4.5-120 (ng/g) were 40.4%-82.0%, 60.6%-86.5%, 69.2%-86.1% and 30.1%-80.3%, respectively. The parent ions and the daughter ions of atrazine, DEA and DIA produced by in source CID were used to identify these chemicals. This method has been successfully applied to the analysis of (atrazine) and its degradation products in environmental soil samples near a pesticide plant.

关 键 词：土壤 除草剂 莠去津 降解产物 提取 高效液相色谱-质谱分析 脱乙基莠去津 脱异丙基莠去津 

分 类 号：S482.4[农业科学—农药学] S481.8[农业科学—植物保护]