毛细管区带电泳测定纳米粒子粒径分布的探索  

Primary Study on Determination of the Size Distribution of Nanoparticles by Capillary Zone Electrophoresis

作　　者：薛艳[1] 杨海鹰[1] 杨永坛[1] 

机构地区：[1]中国石油化工股份有限公司石油化工科学研究院,北京100083

出　　处：《色谱》2004年第2期170-173,共4页Chinese Journal of Chromatography

摘　　要：研究了采用毛细管区带电泳测定纳米粒子粒径分布的可能性。在合适的分散条件下,聚苯乙烯(PS)纳米粒子在进样及电泳迁移过程中不发生团聚。通过选择合适的电泳操作条件,PS纳米粒子可以按照粒径大小得到分离,且迁移时间及峰面积的重现性良好。The separation of polystyrene(PS) latex in sizes of 50 nm and 125 nm has been carried out using capillary zone electrophoresis(CZE). The effects of experimental parameters including pH, the concentration of buffer and the voltage, on the separation were investigated. The optimum electrophoretic conditions were as follows: 8.00 (mmol/L) NaH_2PO_4(adjusted to pH 8.00 with ammonia) as buffer, applied voltage of 26 kV and detection wavelength of 214 nm.The relative standard deviation of the migration time and the peak area of PS50 were 0.3% and 4.7%, respectively. The separation was found to be size-dependent. Larger particles exhibited longer migration time. Stable dispersion condition of PS particles in aqueous solution was obtained by adding PS particles into the buffer.

关 键 词：聚苯乙烯纳米粒子 粒径分布 毛细管区带电泳 分离 纳米材料 

分 类 号：TB383[一般工业技术—材料科学与工程] O657.8[理学—分析化学]