新疆猪瘟病毒分子流行病的研究  

Molecular Epidemiology of Classical Swine Fever Viruses in Xinjiang

作　　者：陈创夫[1] 余兴龙[2] 乔军[3] 孟庆龄[3] 田晶华[1] 王金富[3] 柳建新[1] 张高轩[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]解放军军需大学,吉林长春130062 [3]塔里木农垦大学动物科技学院,新疆阿拉尔843300

出　　处：《石河子大学学报（自然科学版）》2004年第1期56-59,共4页Journal of Shihezi University(Natural Science)

基　　金：新疆生产建设兵团农业科学研究与技术开发计划项目(WKB99TNDNK32XM)

摘　　要：采用RT PCR方法从新疆临床发病猪场采集的病料中扩增了CSFV流行株E2基因的主要抗原位点编码区,测定了15株CSFV的核苷酸序列。应用DNAstar计算机软件对6个地区6个代表株CSFV的核苷酸序列和推导的氨基酸序列进行了比较分析,结果表明:新疆6株CSFV流行株与猪瘟兔化弱毒株(C株)核苷酸序列的同源性为80.9%～82.8%,氨基酸的同源性为86.2%～88.3%,说明新疆CSFV流行株E2基因发生了部分变异,与疫苗株相比抗原性产生一定的差异。Five positive CSFV pathological samples were screened and selected for RT-PCR amplification. The homology of hypervariable regions of E_2 gere of wild strain of CSFV in Xinjiang was 80.9%～82.8% compared with Chinese strain of CSFV.The homology of amino acid sequence was 86.2%～88.3% compared with the chinses CSFV strains mentioned above.The results showed that some difference was found among them,which was greater between strainc and in Xinjiang might bring about significant ontigenic difference.

关 键 词：新疆 猪瘟病毒 分子流行病 抗原性 疫苗株 

分 类 号：S828[农业科学—畜牧学] S855.3[农业科学—畜牧兽医]