九种中药活性成分抗耐药肿瘤细胞体外研究  

In Vitro Research on the Effects Against Drug-Resistant Tumor Cells of Nine Active Components of Traditional Chinese Drugs

作　　者：李峨[1] 陈信义[1] 王玉芝[2] 

机构地区：[1]北京中医药大学东直门医院血液肿瘤科,北京100700 [2]中国中医研究院广安门医院,北京100053

出　　处：《北京中医药大学学报》2004年第2期24-26,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：高等学校博士点专项科研基金项目 (No .2 0 0 0 0 0 2 611) ;国家自然科学基金项目 (No .3 0 2 71670 )

摘　　要：目的　分别从温阳、益气、化痰、活血类中药里选择九种单味中药活性成分 ,拟通过体外细胞培养证实其是否存在抗肿瘤耐药效应。方法　采用MTT比色法 ,选择耐长春新碱的人口腔鳞状上皮癌细胞株 (KBv2 0 0 )为靶细胞 ,观察上述单味中药活性成分细胞毒与增敏效应。结果　桂皮醛、大豆甙元、金雀黄素、薯蓣皂苷对KBv2 0 0 细胞具有明显的抑制效应。结论　桂皮醛、大豆甙元、金雀黄素。Objective To investigate the effects against drug resistant tumor cells of 9 active components of traditional Chinese drugs by cell cultivation. Method MTT colorimetry was used in the detection, and KBv200 was used as the target. The cytotoxicity and enhanced sensitivity of the 9 active components were observed. Result It was found that cinnamic aldehyde, daizein, genistein and dioscin had obvious inhibitory effect on KBv200. Conclusion Cinnamic aldehyde, daizein, genistein and dioscin are worthy of further studies with respect to their inhibition of the growth of tumors.

关 键 词：中药活性成分 肿瘤多药耐药性 人口腔鳞状上皮癌细胞株 体外细胞培养 

分 类 号：R285.5[医药卫生—中药学]