两种贝类基因组DNA的提取及RAPD分析  

The Research on the Genomic DNA Isolation and RAPD Identification of Two Shellfishes

作　　者：王静[1] 高如承[1] 江树勋[2] 骆轩[1] 

机构地区：[1]福建师范大学生物工程学院,福建福州350007 [2]福建出入境检验检疫局技术中心,福建福州350001

出　　处：《福建师范大学学报（自然科学版）》2004年第1期66-69,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省科技攻关计划重大项目(2003N020);科技部农业科技成果转化资金项目(2002350030480);福建省教育厅基金资助项目(JA01022)

摘　　要：研究了福建沿海两种常见贝类波纹巴非蛤(Paphiaundulata)和菲律宾蛤仔(Ruditapesphilip-pinarum)的性腺和斧足的DNA提取方法,并且对它们进行了RAPD分析,结果显示性腺DNA得率较斧足高,并且成功获得了效果较好的2种随机引物及其相应的反应条件,能够在分子水平上为它们及其近源种的比较研究提供初步的资料.Researched on two shellfishes of Paphia (Neotapes) undulata and Ruditapes philippinarum by the molecular level, including the method of genomic DNA isolation and RAPD detection. The result suggested that the yield of DNA from gonad is higher than from pelecypodium. Moreover,two primers had been selected to identify these two shellfishes satisfactorily by RAPD analysis of the extracted genetic DNA. This research provided the basic molecular foundation for further identification of the shellfishes on molecular level.

关 键 词：波纹巴非蛤 菲律宾蛤仔 基因组DNA RAPD 提取方法 随机扩增多态DNA技术 经济贝类 

分 类 号：S968.3[农业科学—水产养殖] Q78[农业科学—水产科学]