运动员尿中类固醇激素水平的研究  

Statistical Analysis of the Urine Steroid Profile in Chinese Athletes

作　　者：王静竹[1] 吴侔天[1] 张亦农[1] 刘欣[1] 杨志勇[1] 

机构地区：[1]国家体育总局运动医学研究所,北京100029

出　　处：《中国运动医学杂志》2004年第2期200-203,共4页Chinese Journal of Sports Medicine

摘　　要：目的 :对 12 17名运动员尿中 8种类固醇激素的含量进行测定及统计分析 ,以考察我国运动员内源性类固醇激素水平。方法 :对尿样进行预提取、水解、萃取、衍生化等前处理 ,采用GC/MS方法分离检测 ,测定内源性类固醇激素含量。结果 :我国男运动员尿中类固醇水平一般高于女性 ,但都普遍低于国外统计数据 ,尤其是睾酮和 5 β雄烷二醇的水平低于国外数据 4～ 7倍。某些类固醇含量的比值 (如An/Etio、T/ET、5α -diol/5 β -diol)较为恒定 。Objective To investigate the steroid levels of urine in Chinese athletes. Methods Subjects were 1217 Chinese athlete, 8 kinds of steroids named androsterone (An), etiocholanolone (Etio), dihydrotestosterone (DHT), dehydroepiandrosterone (DHET), Testosterone (T), Epitestosterone (ET), 5α-androstane-3α, 17β-diol (5α-diol), 5β-androstane-3α, 17β-diol (5β-diol) were measured by gas chromatography combined with mass spectrometry (GC/MS) after the urine sample being treated with chemical pre-extraction, hydrolysis, extraction and derivatization. Results The urine steroid levels of the Chinese male athlete were higher than those of the female athlete, but both male and female athlete had a lower urine steroids especially testosterone and 5β-androstane-3α,17β-diol, 4～7 times lower than other country's athletes. Some ratios of urine steroids such as An/Etio, T/ET, 5α-diol/5β-diol were relatively steady, which were affected less by gender.

关 键 词：运动员 类固醇激素 尿液检测 睾酮 5β雄烷二醇 气相色谱-质谱联用法 

分 类 号：R87[医药卫生—运动医学]