均匀设计在蚕蛹虫草和冬虫夏草活性成分分析中的应用  

Uniform Design Applied to the Determination of Active Components of Cordyceps Militaris and Cordyceps Sinensis

作　　者：周枝凤[1] 梁逸曾[1] 胡芸[1] 郭方遒[1] 

机构地区：[1]中南大学化学化工学院中药现代研究中心,湖南长沙410083

出　　处：《分析测试学报》2004年第2期35-37,40,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金重点项目 (20235020)

摘　　要：采用均匀实验设计方法快速找到蚕蛹虫草活性成分的高效液相色谱最优分离条件 ;将该优化条件用于蚕蛹虫草和冬虫夏草的水溶性成分的分离取得满意结果 ,并对部分组分进行了定性定量分析 ;均匀实验设计方法可较快找到复杂未知或半未知体系的色谱分离条件。The optimal HPLC chromatographic conditions for the separation of active components in Cordyceps militaris were found with the aid of uniform design. As the optimized conditions were applied to separate the water-soluble active constituentes in extracts from Cordyceps militaris and Cordyceps sinensis, satisfactory results were obtained. Both qualitative and quantitative analyses of some active constituents have been conducted on the basis of optimized separation. Hence, uniform design is a good method to optimize the chromatographic separation conditions of complicated systems with unknown or uncertain components.

关 键 词：蚕蛹虫草 冬虫夏草 活性成分 成分分析 均匀设计 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]