低温对小麦花培愈伤组织绿苗分化率的影响  

Effects of cold temperature on the green plantlet differentiation rate of calli in anther culture of wheat

作　　者：宣朴[1] 郭元林[1] 岳春芳[1] 尹春蓉[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066

出　　处：《西南农业学报》2004年第2期146-148,共3页Southwest China Journal of Agricultural Sciences

基　　金：国家小麦辐射育种攻关;四川省农作物育种攻关项目

摘　　要：小麦花药培养的技术关键在于提高花粉植株诱导率。花粉植株诱导率由花药愈伤组织诱导率和愈伤组织绿苗分化率共同决定。本试验以12个不同小麦杂交组合的F2代为材料,研究了在转愈初期短期低温处理对小麦愈伤组织绿苗分化率的影响。结果表明,在转愈伤组织初期,采用了4℃和6℃低温培养1～3d能大幅度提高小麦花培愈伤组织绿苗分化率,提高育种效率。The key to wheat anther culture was to enhance the pollen plantlet production.The effects of cold treatment for the pollen calli on the green plantlet differentiation rate were studied in anther culture of wheat using 12 wheat genotypes.The results showed that the ratio of green plantlet differentiation after 4?℃ and 6?℃ treatments were 26.8?% and 24.2?%,respectively,and were much higher than that of control.

关 键 词：小麦 花药培养 愈伤组织 低温处理 绿苗分化率 杂交组合 单倍体育种 

分 类 号：S512.103.5[农业科学—作物学]