甜蛋白基因MBLII对番茄的遗传转化  

Genetic transformation of sweet protein MBLII on tomato

作　　者：李晓东[1] 刘敬梅[2] 陈杭[3] 程智慧[4] 柴敏[3] 

机构地区：[1]深圳职业技术学院生物应用工程系,广东深圳518055 [2]清华大学生物系,北京100084 [3]北京市农林科学院蔬菜研究中心,北京100089 [4]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2004年第5期808-811,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：北京市科委重点实验室基金资助项目 951890200 ;深圳职业技术学院双高项目 03KJb010 资助

摘　　要：以5～7d龄的 丽春 番茄无菌苗子叶作为外植体,研究了子叶外植体对抗生素卡那霉素的敏感性,抗生素卡那霉素对番茄筛选的适宜浓度为70mg/L.通过根癌农杆菌 Agrobacteriumtumefaciens 介导,成功地进行了马槟榔甜蛋白基因MBLII对番茄的遗传转化,获得转化番茄抗性植株,组织化学法有阳性表现、PCR特异扩增及Southern杂交检测出现特异条带,表明MBLII基因已顺利整合到转基因番茄植株的基因组.The nature sensitive of cotyledon explants on kanamycin were examined,the suitable concentration of kanamycin for selecting transgenic tissue were 70 mg/L for tomato. The stain LBA4404 of Agrobacterium tumerfaciens harboring the binary vector pLX10,which carrying sweet protein gene MBLII,selective gene NPTII and reporter gene GUS, was used to transform cv. Li Chun of tomato. Leaves of transgenic tomato plants were used for assaying the gene existence. Results of histochemical assay、PCR amplification and Southern blotting showed that the MBLII gene was stably integrated into the genome of transgenic tomato plants.

关 键 词：番茄 甜蛋白基因MBLⅡ 遗传转化 卡那霉素 

分 类 号：Q943.2[生物学—植物学]