猪传染性萎缩性鼻炎油佐剂灭活菌苗的研究  

STUDIES ON NEW OIL-ADJUVANT VACCINE FOR INFECTIOUS ATROPHIC RHINITIS OF SWINE

作　　者：彭发泉[1] 杨留战[1] 赵淑春[1] 苑士祥[1] 张树成[1] 杨旭夫[1] 洪秀聪[1] 王振华[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,哈尔滨150001

出　　处：《中国农业科学》1992年第2期1-13,共13页Scientia Agricultura Sinica

摘　　要：以Bb I相菌液和司盘85为乳化剂研制的油佐剂福年马林灭活菌苗——S85油苗,无论母猪注苗、仔猪注苗或母-仔注苗,均安全有效。妊娠母猪一次注苗,初乳K凝集价平均在10万倍以上,所生仔猪于3-7日龄血清K凝集价平均4万倍以上,攻毒后鼻甲骨萎缩平均减少率(TAD)为95.5%,而攻击的对照猪平均病变率91%,3/4为明显至严重萎缩。非注苗母猪产仔猪于1及3-4周龄注苗2次,对攻击菌的清菌率100%,注苗或未注苗母猪所产仔猪于3-4周龄注苗1次,清菌率80%-90%,同时湿苗滴鼻2次,可明显提高Bb指数减少率。注苗母猪所产仔猪于3-4周龄及8周龄各注苗1次,不发生感染。菌苗在4℃保存2年效力不变。仔猪TAD明显高于4种国外商品苗。在8个AR猪场应用,于7-8周龄时Bb感染减少率在单纯母猪注苗为70%-80%,母-仔注苗92%以上,连续三产母-仔注苗,每产在10-12周龄时为97%以上。在单纯母猪注苗(一产)场屠宰时AR病变减少90%,而对照群病变率65%-87%,1/2或2/3为明显萎缩:屠宰日增重比未注苗猪提高5.8%-13.2%。A new oil-adjuvant vaccine of formalinized phase I Bordetella bronchiseptica (Bb), using span 85 as emulsifier, named S85 oil-vaccine AR, was developed for controlling infectious atrophic rhinitis of swine (AR). Its potency was greatly superior to that of oil-vaccine with span 80 and aluminium hydroxide gel absorbed vaccine. It was safe and effective in sow-vaccination, piglet-vaccination and sow-piglet-vaccination. Single and double vaccinations of pregnant sows were equally potent, the mean titre of K agglut inin in colustra exceeded 1:100000, the mean titre of serum K agglutinin of piglets at 3 to 7 days exceeded 1:40000, and the mean percentage of turbinate atrophy rate decrease (TAD%) of litters challenged at this range of age was 95.5% with only mild TA cases, whereas the mean TA% of control litters was 91% with marked to severe atrophy in three-fourths of the cases. Duration of passive immunity against Bb infection lasted for approximate 1.5-2 months when piglets were challenged with low doses of Bb or in AR herds exposed to natural infection. Litters of unvaccinated sows vaccinated twice at one week and 3-4 wks showed 100% of clearance rate (CR%) of inoculated Bb. Litters of vaccinated or unvaccinated sows vaccinated once at 3-4 wks showed 80-90% of CR, which in combination of intranasal instillation of wet.vac cine further raised Bb index decrease ( BID )%. Litters of vaccinated sow vaccin ated twice at 3-4 wks and 8 wks showed no colonization of Bb pi. No decrease of potency occurred during two-year storage in refrigerator. In comparative experiments of sow-vaccination, evaluated by TAD% of piglets of sow vaccinated twice, NobivacAR T (Intervet) was 86.7%, Kitasato AR vaccine 41.2%, Nobivac AR (Intervet) 21%, and AR-PAC-P(Schering)16.7%, and of sows vaccinated once Nobivac AR-T was 40%. of the last four groups, a part or more than half showed marked TA, and the mean titers of K agglutinin in colostra were 4 to 400 times less than those of S85 oil-vaccine AR. In field studies of S85 oil-vaccine AR on 8 A

关 键 词：猪 S85油苗 传染性 萎缩性 鼻炎 

分 类 号：S858.287.7[农业科学—临床兽医学]