白头翁体外对TNF、IL-1、IL-6产生的影响  

The effects of the root of chinese pulsatilla on TNF,IL-1and IL-6 in vitro

作　　者：杨昆[1] 李培凡[2] 肖向茜[3] 

机构地区：[1]天津医科大学实验中心,天津300070 [2]天津医学高等专科学校 [3]天津医科大学药理学教研室

出　　处：《天津医科大学学报》2004年第1期59-61,共3页Journal of Tianjin Medical University

摘　　要：目的 :观察白头翁对抑制脂多糖(LPS)刺激肝枯否氏细胞(KC)分泌肿瘤坏死因子(TNF)、白细胞介素1(IL 1)和白细胞介素6(IL 6)的影响。方法:大鼠KC培养24h后,加入24孔板内(细胞终浓度为1×106 个/ml)静置4h。分4组:空白对照组:KC培养液 +RPMI1640。LPS组:KS培养液 +LPS(终浓度为20μg/ml) +RPMI1640。白头翁Ⅰ组:KC培养液 +LPS(终浓度为20μg/ml) +白头翁(终浓度为100μg/ml)。置37℃、5 %CO2 孵育箱中培养2、4、6、8、12、24h后取上清液,分别测定TNF、IL 1、IL 6的活性。结果:白头翁能明显抑制LPS刺激肝KC分泌TNF、IL 1和IL 6,且这种抑制作用随培养时间的延长而增强,并与药物浓度有关。结论:白头翁可能通过抑制LPS刺激肝KC分泌TNF、IL 1和ILObjective:To observe the effects of the root of Chinese Pulsatilla(RCP)on the secretion of TNF,ILˉ1and ILˉ6from Kupffer cells(KC)stimulated by Lipopolysaccharide(LPS).Methods:The rat hepatic Kupffer cells were cultivated for24hours in the24ˉcaveˉboard(the final cellular concentration was1×10 6 /ml)and then incubated for4hours.The samples were divided into4groups:(1)the control group:the KC cultre medium+RPMI1640.(2)The LPS group:the KC culture medium+LPS(the final concentration was20μg/ml)+RPMI1640.(3)The first RCP group:the KC culture medium+LPS(the final concentration was20μg/ml)+RCP(the final concentration was100μg/ml).(4)The second RCP group:the KC culture medium+LPS(the final concentration was20μg/ml)+RCP(the final concentration was10μg/ml).After the cells were incubated for2,4,6,8,12and24hours,the supernatant was collected to assay the activity of TNF,ILˉ1and ILˉ6,respectively.Results:The RCP strongly inhibits the secretion of TNF,ILˉ1and ILˉ6from KC stimulated by LPS.This effect increases with time and concentration of the drug,Conclusion:One of the antiˉinflammatory mechanisms of RCP is possibly that it can suppress the secretion of TNF,ILˉ1and ILˉ6from KC stimulated by LPS.

关 键 词：脂多糖 枯否氏细胞 细胞因子 白头翁 

分 类 号：R282.71[医药卫生—中药学] R392.11[医药卫生—中医学]