补肾通络益智方对血管性痴呆大鼠脑内氧自由基代谢影响的实验研究  

The Experimental Study about the Effect of Bushentongluoyizhi Decoction on the Metabolism Oxygen-derived Free Radidicals in the Brain of Rats with Vascular Dementia

作　　者：王风雷[1] 张炜宁 孙兆泉[1] 金幼兰[1] 张崇泉 

机构地区：[1]湖南省中医药研究院附属医院,湖南长沙410006 [2]深圳市福田区中医院,广东深圳518034

出　　处：《湖南中医药导报》2004年第4期65-67,共3页Hunan Guiding Journal of Traditional Chinese Medicine and Pharmacology

摘　　要：目的 :观察中药复方补肾通络益智方 (BSTLYZ)对脑血管梗塞性痴呆大鼠脑部氧自由基代谢的影响 ,探讨该方药治疗血管性痴呆的作用机理。方法 :经颈总动脉暂时夹闭加颈外动脉注射栓子方法造成大鼠多发性脑梗塞 ,术后加皮下注射山莨菪碱以促进智力障碍形成。以记忆力试验检测模型 ,并测量各组大鼠脑内皮层、下丘脑、海马、纹状体部超氧化物歧化酶 (SOD)活性及过氧化脂质 (LPO)含量。结果 :补肾通络益智方给药组大鼠学习记忆力能力明显增强 ,脑四部位SOD活性有不同程度增加 ,LPO含量下降 ,两指标变化与模型对照组比较 ,差异均有统计学意义 (P <0 .0 1或P <0 .0 5 ) ,尤以对大脑皮层作用明显强于对照药三乐喜 (P <0 .0 1) ,补肾通络益智方对大脑其余三部位SOD和LPO的影响与三乐喜相当 (P >0 .0 5 )。结论 :补肾通络益智方具有提高模型动物脑内氧自由基代谢能力 ,为临床治疗血管性痴呆提供了一定的实验依据。Objective: To observe the effect of Bushentongluoyizhi Decoction on the metabolism oxygen-derived free radidicals in the brain of rats with vascular dementia, and discussion on the mechanism of the decoction for the treatment of vascular dementia. Methods: The rats model of cerebral infarction was established by combination of common carotid artery closure by clip and injecting emboli in external carotid artery, promoting the anisodamine by dysnoesia subcutaneous injection. The activity of SOD and the content of LPO were detected in endodermis of brain, hypothalami, hippocampi and striatum with the anamnesis tests model. Results: It was distinctly improved of anamnesis in Bushentongluoyizhi Decoction with the improvement of the activity of SOD and the descent of LPO contents, which has statistics significance compared to control group (P< 0.01 or P< 0.05 ), especially the effect on cerebral cortex better than Aniracetam (P< 0.01 ), which the effect on other 3 spots were similar(P> 0.05 ). Conclusion: Bushentongluoyizhi Decoction could improve the metabolism of free radical, which provide experiment basement for the treatment of vascular dementia.

关 键 词：补肾通络益智方 血管性痴呆 大鼠 超氧化物歧化酶 过氧化脂质 

分 类 号：R285.5[医药卫生—中药学]