黄豆脲酶的提取及其在缓释肥料中尿素态氮测定中的应用  

The Extraction of Urease from Glycine Max and Its Application to the Determination of Urea-Nitrogen in Controlled Release Fertilizers

作　　者：邹菁[1] 喻德忠[1] 杨先进 孟艳华[1] 白雪石[1] 

机构地区：[1]武汉化工学院制药工程系 [2]武汉云宏农化科技发展有限公司,武汉430073

出　　处：《分析科学学报》2004年第2期178-180,共3页Journal of Analytical Science

基　　金：湖北省教育厅重点科技项目(No.2001A20008)

摘　　要：本文建立了准确、快速、经济的控制缓释肥料中尿素态氮的测定方法。采用自制黄豆脲酶水解液,于pH7.0的磷酸盐缓冲体系中水解尿素,以奈氏试剂为显色剂,用分光光度法测定尿素态氮。该方法在0～2.50μg/mL范围内符合比耳定律,检出限为0.01μg/mL,用于控制缓释肥料中尿素态氮的测定,回收率在96.2%到100.8%之间,与商品脲酶测定结果接近。A accurate, rapid and economic method for the determination of urea-nitrogen in controlled release fertilizers was developed. The urea was hydrolysised in (pH 7.0) buffer medium, and the urea-nitrogen was determined by Spectrophotometry with Nessler's reagent as the chromogenic reagent. Beer's law is obeyed in the range of 0～2.50 μg/mL with λ_(max)=420 nm. The detection limit is 0.01 μg/ml. This assay was applied to determinate urea-nitrogen in controlled release fertilizers with recovery ranging from 96.2% to 100.8%.

关 键 词：黄豆脲酶 提取 脲醛控制缓释肥料 尿素态氮 测定 分光光度法 质量控制 

分 类 号：TQ441.4[化学工程—化学肥料工业] TQ449.1