冠心病不同类型间T细胞增殖反应的比较研究  

Comparative study of T cell proliferative response in different types of coronary heart diseases

作　　者：郑华[1] 吴赛珠[1] 赖文岩[1] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515

出　　处：《第一军医大学学报》2004年第4期386-388,共3页Journal of First Military Medical University

基　　金："973"国家重点基础研究发展规划(G200057008)~~

摘　　要：目的研究T细胞增殖反应与冠心病斑块稳定性的关系。方法采用MTS/PMS比色分析法测定13例急性心肌梗死、22例不稳定性心绞痛、15例稳定性心绞痛和16例对照者的T细胞对植物血凝素(PHA)、氧化低密度脂蛋白(oxLDL)及低密度脂蛋白(LDL)的增殖反应。结果急性心肌梗死组和不稳定性心绞痛组的T细胞对PHA的增殖反应高于稳定性心绞痛组和对照组(P<0.05),急性心肌梗死组和不稳定性心绞痛组的T细胞对5和1 μg/ml oxLDL的增殖反应高于稳定性心绞痛组和对照组(P<0.05),急性心肌梗死组和不稳定性心绞痛组的T细胞对3种浓度(10、5和1 μg/ml)oxLDL的增殖反应均高于对同浓度的LDL的增殖反应(P<0.05)。结论T细胞介导的细胞免疫,尤其是对特异性抗原oxLDL的免疫反应,在导致斑块的不稳定及急性冠状动脉综合征的发生中可能起着重要的作用。Objective To investigate the association between proliferative response of T cells and plaque stability in coronary heart disease. Methods A total of 66 subjects were recruited in the study, including 13 patients with acute myocardial infarction (AMI), 22 with unstable angina pectoris (UAP), 15 with stable angina pectoris (SAP) and 16 control subjects without coronary heart disease. The proliferative response of T cells to phytohemagglutinin (PHA), oxidized low-density lipoprotein (oxLDL) and low-density lipoprotein (LDL) was examined by MTS/PMS colorimetric assay. Results Significantly stronger proliferative response of T cells to PHA was noted in AMI and UAP groups than in SAP and control groups (P<0.05), as with the response to oxLDL (at doses of 5 and 1 μg/ml, P<0.05). T cell proliferative response to oxLDL (at the doses of 10, 5 and 1 μg/ml) appeared significantly stronger than that to LDL (at the same dose as oxLDL) in AMI group and UAP group (P<0.05). Conclusion Cellular immunity mediated by T cells, especially the immune response to oxLDL, may play an important role in the instability of plaque and the occurrence of acute coronary syndrome (ACS).

关 键 词：冠心病 T细胞 增殖反应 比较研究 氧化低密度脂蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]